Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14505 and SGL_RS14510 are separated by 125 nucleotides SGL_RS14510 and SGL_RS14515 are separated by 135 nucleotides SGL_RS14515 and SGL_RS14520 overlap by 4 nucleotides SGL_RS14520 and SGL_RS14525 are separated by 102 nucleotides
SGL_RS14505: SGL_RS14505 - glutamate-5-semialdehyde dehydrogenase, at 2,709,084 to 2,710,385
_RS14505
SGL_RS14510: SGL_RS14510 - DUF3177 family protein, at 2,710,511 to 2,711,107
_RS14510
SGL_RS14515: SGL_RS14515 - type II toxin-antitoxin system HicA family toxin, at 2,711,243 to 2,711,422
_RS14515
SGL_RS14520: SGL_RS14520 - type II toxin-antitoxin system HicB family antitoxin, at 2,711,419 to 2,711,637
_RS14520
SGL_RS14525: SGL_RS14525 - reverse transcriptase domain-containing protein, at 2,711,740 to 2,713,305
_RS14525
Position (kb)
2710
2711
2712 Strain fitness (log2 ratio)
-2
-1
0
1 at 2709.540 kb on + strand, within SGL_RS14505 at 2709.554 kb on - strand, within SGL_RS14505 at 2709.649 kb on + strand, within SGL_RS14505 at 2709.671 kb on - strand, within SGL_RS14505 at 2709.745 kb on - strand, within SGL_RS14505 at 2710.087 kb on - strand, within SGL_RS14505 at 2710.178 kb on - strand, within SGL_RS14505 at 2710.380 kb on + strand at 2710.439 kb on - strand at 2710.489 kb on + strand at 2710.497 kb on - strand at 2710.650 kb on + strand, within SGL_RS14510 at 2710.652 kb on + strand, within SGL_RS14510 at 2710.660 kb on - strand, within SGL_RS14510 at 2710.722 kb on - strand, within SGL_RS14510 at 2711.162 kb on + strand at 2711.455 kb on + strand, within SGL_RS14520 at 2711.505 kb on - strand, within SGL_RS14520 at 2712.000 kb on - strand, within SGL_RS14525
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,709,540 + SGL_RS14505 0.35 -1.0 2,709,554 - SGL_RS14505 0.36 -0.3 2,709,649 + SGL_RS14505 0.43 +0.3 2,709,671 - SGL_RS14505 0.45 +0.6 2,709,745 - SGL_RS14505 0.51 +1.1 2,710,087 - SGL_RS14505 0.77 -0.4 2,710,178 - SGL_RS14505 0.84 -1.3 2,710,380 + -0.1 2,710,439 - -1.9 2,710,489 + -0.0 2,710,497 - +0.1 2,710,650 + SGL_RS14510 0.23 -1.2 2,710,652 + SGL_RS14510 0.24 -2.1 2,710,660 - SGL_RS14510 0.25 -2.3 2,710,722 - SGL_RS14510 0.35 +0.1 2,711,162 + -1.3 2,711,455 + SGL_RS14520 0.16 -0.0 2,711,505 - SGL_RS14520 0.39 +0.1 2,712,000 - SGL_RS14525 0.17 +0.0
Or see this region's nucleotide sequence