Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14415 and SGL_RS14420 overlap by 1 nucleotides SGL_RS14420 and SGL_RS14425 are separated by 82 nucleotides SGL_RS14425 and SGL_RS14430 overlap by 79 nucleotides SGL_RS14430 and SGL_RS14435 are separated by 2 nucleotides
SGL_RS14415: SGL_RS14415 - DNA topoisomerase (ATP-hydrolyzing) subunit A, at 2,687,952 to 2,690,534
_RS14415
SGL_RS14420: SGL_RS14420 - TIGR04168 family protein, at 2,690,534 to 2,691,445
_RS14420
SGL_RS14425: SGL_RS14425 - energy-coupling factor ABC transporter ATP-binding protein, at 2,691,528 to 2,692,382
_RS14425
SGL_RS14430: SGL_RS14430 - cobalt ECF transporter T component CbiQ, at 2,692,304 to 2,693,077
_RS14430
SGL_RS14435: SGL_RS14435 - cobalt transporter CbiM, at 2,693,080 to 2,693,724
_RS14435
Position (kb)
2691
2692
2693 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2690.528 kb on + strand at 2690.538 kb on + strand at 2690.564 kb on - strand at 2690.639 kb on - strand, within SGL_RS14420 at 2690.825 kb on + strand, within SGL_RS14420 at 2690.892 kb on + strand, within SGL_RS14420 at 2690.901 kb on - strand, within SGL_RS14420 at 2690.946 kb on - strand, within SGL_RS14420 at 2691.141 kb on - strand, within SGL_RS14420 at 2691.171 kb on - strand, within SGL_RS14420 at 2691.194 kb on - strand, within SGL_RS14420 at 2691.274 kb on - strand, within SGL_RS14420 at 2691.382 kb on + strand at 2691.769 kb on - strand, within SGL_RS14425 at 2691.769 kb on - strand, within SGL_RS14425 at 2691.848 kb on - strand, within SGL_RS14425 at 2692.017 kb on + strand, within SGL_RS14425 at 2692.034 kb on + strand, within SGL_RS14425 at 2692.146 kb on + strand, within SGL_RS14425 at 2692.220 kb on - strand, within SGL_RS14425 at 2692.317 kb on - strand at 2692.364 kb on + strand at 2692.372 kb on - strand at 2692.440 kb on + strand, within SGL_RS14430 at 2692.440 kb on + strand, within SGL_RS14430 at 2692.468 kb on + strand, within SGL_RS14430 at 2692.476 kb on - strand, within SGL_RS14430 at 2692.476 kb on - strand, within SGL_RS14430 at 2692.543 kb on + strand, within SGL_RS14430 at 2692.551 kb on - strand, within SGL_RS14430 at 2692.564 kb on - strand, within SGL_RS14430 at 2692.645 kb on - strand, within SGL_RS14430 at 2692.970 kb on + strand, within SGL_RS14430 at 2693.046 kb on + strand at 2693.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,690,528 + -0.1 2,690,538 + +0.0 2,690,564 - -0.1 2,690,639 - SGL_RS14420 0.12 -0.0 2,690,825 + SGL_RS14420 0.32 -0.3 2,690,892 + SGL_RS14420 0.39 +0.3 2,690,901 - SGL_RS14420 0.40 -0.3 2,690,946 - SGL_RS14420 0.45 -0.2 2,691,141 - SGL_RS14420 0.67 -0.2 2,691,171 - SGL_RS14420 0.70 -0.1 2,691,194 - SGL_RS14420 0.72 -0.3 2,691,274 - SGL_RS14420 0.81 +0.7 2,691,382 + +0.2 2,691,769 - SGL_RS14425 0.28 -1.7 2,691,769 - SGL_RS14425 0.28 -4.1 2,691,848 - SGL_RS14425 0.37 -3.0 2,692,017 + SGL_RS14425 0.57 -4.5 2,692,034 + SGL_RS14425 0.59 -2.5 2,692,146 + SGL_RS14425 0.72 -2.0 2,692,220 - SGL_RS14425 0.81 -2.6 2,692,317 - +0.3 2,692,364 + -3.5 2,692,372 - -3.6 2,692,440 + SGL_RS14430 0.18 -2.7 2,692,440 + SGL_RS14430 0.18 -0.9 2,692,468 + SGL_RS14430 0.21 -1.9 2,692,476 - SGL_RS14430 0.22 -1.5 2,692,476 - SGL_RS14430 0.22 -1.3 2,692,543 + SGL_RS14430 0.31 -4.0 2,692,551 - SGL_RS14430 0.32 -3.2 2,692,564 - SGL_RS14430 0.34 -3.5 2,692,645 - SGL_RS14430 0.44 -3.0 2,692,970 + SGL_RS14430 0.86 -3.3 2,693,046 + -2.9 2,693,067 + -1.1
Or see this region's nucleotide sequence