Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS19040 and SGL_RS14100 overlap by 14 nucleotides SGL_RS14100 and SGL_RS14105 are separated by 38 nucleotides SGL_RS14105 and SGL_RS14110 are separated by 187 nucleotides SGL_RS14110 and SGL_RS14115 overlap by 4 nucleotides
SGL_RS19040: SGL_RS19040 - hypothetical protein, at 2,622,526 to 2,622,981
_RS19040
SGL_RS14100: SGL_RS14100 - gamma-glutamylcyclotransferase, at 2,622,968 to 2,623,402
_RS14100
SGL_RS14105: SGL_RS14105 - 2-succinylbenzoate--CoA ligase, at 2,623,441 to 2,624,907
_RS14105
SGL_RS14110: SGL_RS14110 - phosphomannose isomerase type II C-terminal cupin domain, at 2,625,095 to 2,625,481
_RS14110
SGL_RS14115: SGL_RS14115 - NAD(P)-dependent oxidoreductase, at 2,625,478 to 2,626,479
_RS14115
Position (kb)
2623
2624
2625 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2622.544 kb on + strand at 2622.628 kb on - strand, within SGL_RS19040 at 2622.631 kb on - strand, within SGL_RS19040 at 2622.748 kb on + strand, within SGL_RS19040 at 2622.840 kb on - strand, within SGL_RS19040 at 2622.966 kb on - strand at 2623.080 kb on + strand, within SGL_RS14100 at 2623.085 kb on + strand, within SGL_RS14100 at 2623.236 kb on - strand, within SGL_RS14100 at 2623.247 kb on + strand, within SGL_RS14100 at 2623.370 kb on - strand at 2623.404 kb on - strand at 2624.028 kb on - strand, within SGL_RS14105 at 2624.052 kb on - strand, within SGL_RS14105 at 2624.055 kb on + strand, within SGL_RS14105 at 2624.130 kb on - strand, within SGL_RS14105 at 2624.147 kb on + strand, within SGL_RS14105 at 2624.147 kb on + strand, within SGL_RS14105 at 2624.256 kb on - strand, within SGL_RS14105 at 2624.335 kb on - strand, within SGL_RS14105 at 2624.335 kb on - strand, within SGL_RS14105 at 2624.736 kb on - strand, within SGL_RS14105 at 2624.752 kb on - strand, within SGL_RS14105 at 2624.781 kb on - strand at 2624.910 kb on + strand at 2625.020 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,622,544 + -0.2 2,622,628 - SGL_RS19040 0.22 +0.1 2,622,631 - SGL_RS19040 0.23 +0.3 2,622,748 + SGL_RS19040 0.49 +0.5 2,622,840 - SGL_RS19040 0.69 +0.2 2,622,966 - -0.0 2,623,080 + SGL_RS14100 0.26 +0.3 2,623,085 + SGL_RS14100 0.27 -0.3 2,623,236 - SGL_RS14100 0.62 +0.2 2,623,247 + SGL_RS14100 0.64 -0.3 2,623,370 - +0.1 2,623,404 - +0.2 2,624,028 - SGL_RS14105 0.40 -3.2 2,624,052 - SGL_RS14105 0.42 -3.3 2,624,055 + SGL_RS14105 0.42 -2.9 2,624,130 - SGL_RS14105 0.47 -0.9 2,624,147 + SGL_RS14105 0.48 -1.9 2,624,147 + SGL_RS14105 0.48 -3.3 2,624,256 - SGL_RS14105 0.56 -3.3 2,624,335 - SGL_RS14105 0.61 -2.7 2,624,335 - SGL_RS14105 0.61 -3.2 2,624,736 - SGL_RS14105 0.88 -2.2 2,624,752 - SGL_RS14105 0.89 -3.3 2,624,781 - -2.7 2,624,910 + -0.7 2,625,020 - -1.1
Or see this region's nucleotide sequence