Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13580 and SGL_RS13585 are separated by 136 nucleotides SGL_RS13585 and SGL_RS13590 overlap by 28 nucleotides
SGL_RS13580: SGL_RS13580 - FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO, at 2,516,814 to 2,518,184
_RS13580
SGL_RS13585: SGL_RS13585 - PAS domain S-box protein, at 2,518,321 to 2,521,857
_RS13585
SGL_RS13590: SGL_RS13590 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG, at 2,521,830 to 2,523,737
_RS13590
Position (kb)
2518
2519
2520
2521
2522 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2517.420 kb on + strand, within SGL_RS13580 at 2517.433 kb on + strand, within SGL_RS13580 at 2517.441 kb on - strand, within SGL_RS13580 at 2517.561 kb on - strand, within SGL_RS13580 at 2517.691 kb on - strand, within SGL_RS13580 at 2517.765 kb on - strand, within SGL_RS13580 at 2517.793 kb on - strand, within SGL_RS13580 at 2517.847 kb on - strand, within SGL_RS13580 at 2517.903 kb on + strand, within SGL_RS13580 at 2517.903 kb on + strand, within SGL_RS13580 at 2517.909 kb on + strand, within SGL_RS13580 at 2518.031 kb on + strand, within SGL_RS13580 at 2518.034 kb on - strand, within SGL_RS13580 at 2518.055 kb on + strand at 2518.140 kb on + strand at 2518.165 kb on - strand at 2518.169 kb on + strand at 2518.177 kb on - strand at 2518.249 kb on - strand at 2518.268 kb on - strand at 2518.310 kb on + strand at 2518.386 kb on + strand at 2518.436 kb on + strand at 2519.359 kb on - strand, within SGL_RS13585 at 2519.409 kb on + strand, within SGL_RS13585 at 2519.419 kb on - strand, within SGL_RS13585 at 2519.948 kb on + strand, within SGL_RS13585 at 2519.948 kb on + strand, within SGL_RS13585 at 2519.951 kb on - strand, within SGL_RS13585 at 2519.956 kb on - strand, within SGL_RS13585 at 2519.956 kb on - strand, within SGL_RS13585 at 2520.072 kb on - strand, within SGL_RS13585 at 2520.296 kb on - strand, within SGL_RS13585 at 2520.531 kb on + strand, within SGL_RS13585 at 2520.539 kb on + strand, within SGL_RS13585 at 2520.573 kb on - strand, within SGL_RS13585 at 2520.943 kb on - strand, within SGL_RS13585 at 2521.082 kb on - strand, within SGL_RS13585 at 2521.470 kb on + strand, within SGL_RS13585 at 2521.553 kb on - strand at 2521.696 kb on + strand at 2521.707 kb on + strand at 2521.791 kb on - strand at 2521.808 kb on - strand at 2522.314 kb on + strand, within SGL_RS13590 at 2522.807 kb on + strand, within SGL_RS13590
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,517,420 + SGL_RS13580 0.44 +0.1 2,517,433 + SGL_RS13580 0.45 -0.3 2,517,441 - SGL_RS13580 0.46 +0.6 2,517,561 - SGL_RS13580 0.54 -0.3 2,517,691 - SGL_RS13580 0.64 +0.0 2,517,765 - SGL_RS13580 0.69 -0.1 2,517,793 - SGL_RS13580 0.71 +0.5 2,517,847 - SGL_RS13580 0.75 +0.0 2,517,903 + SGL_RS13580 0.79 +0.2 2,517,903 + SGL_RS13580 0.79 +0.1 2,517,909 + SGL_RS13580 0.80 -0.4 2,518,031 + SGL_RS13580 0.89 +0.4 2,518,034 - SGL_RS13580 0.89 -0.5 2,518,055 + +0.4 2,518,140 + -0.1 2,518,165 - +0.2 2,518,169 + -0.6 2,518,177 - +0.0 2,518,249 - -1.8 2,518,268 - -1.8 2,518,310 + -1.1 2,518,386 + -2.3 2,518,436 + -3.9 2,519,359 - SGL_RS13585 0.29 -3.6 2,519,409 + SGL_RS13585 0.31 -2.6 2,519,419 - SGL_RS13585 0.31 -4.1 2,519,948 + SGL_RS13585 0.46 -3.2 2,519,948 + SGL_RS13585 0.46 -2.0 2,519,951 - SGL_RS13585 0.46 -3.5 2,519,956 - SGL_RS13585 0.46 -3.3 2,519,956 - SGL_RS13585 0.46 -3.2 2,520,072 - SGL_RS13585 0.50 -3.2 2,520,296 - SGL_RS13585 0.56 +0.2 2,520,531 + SGL_RS13585 0.62 -3.5 2,520,539 + SGL_RS13585 0.63 -2.9 2,520,573 - SGL_RS13585 0.64 -2.6 2,520,943 - SGL_RS13585 0.74 -2.8 2,521,082 - SGL_RS13585 0.78 -1.4 2,521,470 + SGL_RS13585 0.89 -2.6 2,521,553 - -2.5 2,521,696 + -2.7 2,521,707 + -3.6 2,521,791 - -3.0 2,521,808 - -1.2 2,522,314 + SGL_RS13590 0.25 +0.8 2,522,807 + SGL_RS13590 0.51 -1.4
Or see this region's nucleotide sequence