Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12975 and SGL_RS12985 are separated by 255 nucleotides SGL_RS12985 and SGL_RS19465 are separated by 15 nucleotides SGL_RS19465 and SGL_RS12990 are separated by 30 nucleotides SGL_RS12990 and SGL_RS12995 overlap by 1 nucleotides
SGL_RS12975: SGL_RS12975 - glycosyltransferase family 1 protein, at 2,385,939 to 2,387,168
_RS12975
SGL_RS12985: SGL_RS12985 - decarboxylating NADP(+)-dependent phosphogluconate dehydrogenase, at 2,387,424 to 2,388,851
_RS12985
SGL_RS19465: SGL_RS19465 - hypothetical protein, at 2,388,867 to 2,389,031
_RS19465
SGL_RS12990: SGL_RS12990 - hypothetical protein, at 2,389,062 to 2,389,481
_RS12990
SGL_RS12995: SGL_RS12995 - hypothetical protein, at 2,389,481 to 2,389,900
_RS12995
Position (kb)
2387
2388
2389 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2387.263 kb on - strand at 2387.272 kb on - strand at 2387.462 kb on + strand at 2387.462 kb on + strand at 2387.474 kb on + strand at 2387.869 kb on + strand, within SGL_RS12985 at 2387.903 kb on + strand, within SGL_RS12985 at 2387.919 kb on - strand, within SGL_RS12985 at 2387.969 kb on + strand, within SGL_RS12985 at 2388.431 kb on + strand, within SGL_RS12985 at 2388.524 kb on - strand, within SGL_RS12985 at 2388.759 kb on - strand at 2388.875 kb on - strand at 2389.091 kb on - strand at 2389.124 kb on + strand, within SGL_RS12990 at 2389.425 kb on + strand, within SGL_RS12990 at 2389.565 kb on + strand, within SGL_RS12995 at 2389.586 kb on + strand, within SGL_RS12995 at 2389.609 kb on + strand, within SGL_RS12995 at 2389.715 kb on - strand, within SGL_RS12995
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,387,263 - +0.1 2,387,272 - -0.3 2,387,462 + -2.6 2,387,462 + -0.7 2,387,474 + -3.2 2,387,869 + SGL_RS12985 0.31 -1.8 2,387,903 + SGL_RS12985 0.34 +0.5 2,387,919 - SGL_RS12985 0.35 -1.3 2,387,969 + SGL_RS12985 0.38 -1.2 2,388,431 + SGL_RS12985 0.71 -0.5 2,388,524 - SGL_RS12985 0.77 -2.0 2,388,759 - -2.2 2,388,875 - -0.1 2,389,091 - -0.1 2,389,124 + SGL_RS12990 0.15 -0.0 2,389,425 + SGL_RS12990 0.86 +0.0 2,389,565 + SGL_RS12995 0.20 -0.0 2,389,586 + SGL_RS12995 0.25 -0.8 2,389,609 + SGL_RS12995 0.30 +0.3 2,389,715 - SGL_RS12995 0.56 -0.1
Or see this region's nucleotide sequence