Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12655 and SGL_RS12660 are separated by 26 nucleotides SGL_RS12660 and SGL_RS12665 are separated by 35 nucleotides SGL_RS12665 and SGL_RS12675 are separated by 252 nucleotides
SGL_RS12655: SGL_RS12655 - hypothetical protein, at 2,295,152 to 2,297,341
_RS12655
SGL_RS12660: SGL_RS12660 - DNA double-strand break repair nuclease NurA, at 2,297,368 to 2,298,570
_RS12660
SGL_RS12665: SGL_RS12665 - hypothetical protein, at 2,298,606 to 2,299,118
_RS12665
SGL_RS12675: SGL_RS12675 - Gfo/Idh/MocA family oxidoreductase, at 2,299,371 to 2,300,441
_RS12675
Position (kb)
2297
2298
2299 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2296.625 kb on + strand, within SGL_RS12655 at 2296.633 kb on - strand, within SGL_RS12655 at 2296.824 kb on - strand, within SGL_RS12655 at 2296.936 kb on - strand, within SGL_RS12655 at 2297.028 kb on + strand, within SGL_RS12655 at 2297.100 kb on - strand, within SGL_RS12655 at 2297.113 kb on - strand, within SGL_RS12655 at 2297.164 kb on - strand at 2297.203 kb on - strand at 2297.338 kb on - strand at 2297.408 kb on + strand at 2297.409 kb on + strand at 2297.635 kb on + strand, within SGL_RS12660 at 2297.635 kb on + strand, within SGL_RS12660 at 2297.643 kb on - strand, within SGL_RS12660 at 2297.672 kb on - strand, within SGL_RS12660 at 2297.737 kb on + strand, within SGL_RS12660 at 2297.885 kb on + strand, within SGL_RS12660 at 2297.885 kb on + strand, within SGL_RS12660 at 2297.893 kb on - strand, within SGL_RS12660 at 2297.972 kb on - strand, within SGL_RS12660 at 2298.047 kb on + strand, within SGL_RS12660 at 2298.054 kb on + strand, within SGL_RS12660 at 2298.087 kb on - strand, within SGL_RS12660 at 2298.103 kb on + strand, within SGL_RS12660 at 2298.172 kb on - strand, within SGL_RS12660 at 2298.205 kb on + strand, within SGL_RS12660 at 2298.213 kb on - strand, within SGL_RS12660 at 2298.264 kb on + strand, within SGL_RS12660 at 2298.380 kb on + strand, within SGL_RS12660 at 2298.380 kb on + strand, within SGL_RS12660 at 2298.380 kb on + strand, within SGL_RS12660 at 2298.388 kb on - strand, within SGL_RS12660 at 2298.388 kb on - strand, within SGL_RS12660 at 2298.567 kb on + strand at 2298.776 kb on + strand, within SGL_RS12665 at 2298.784 kb on - strand, within SGL_RS12665 at 2298.819 kb on - strand, within SGL_RS12665 at 2298.819 kb on - strand, within SGL_RS12665 at 2298.856 kb on - strand, within SGL_RS12665 at 2298.857 kb on + strand, within SGL_RS12665 at 2298.876 kb on - strand, within SGL_RS12665 at 2298.925 kb on + strand, within SGL_RS12665 at 2299.031 kb on - strand, within SGL_RS12665 at 2299.070 kb on - strand at 2299.103 kb on - strand at 2299.120 kb on + strand at 2299.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,296,625 + SGL_RS12655 0.67 -0.9 2,296,633 - SGL_RS12655 0.68 -0.5 2,296,824 - SGL_RS12655 0.76 +0.6 2,296,936 - SGL_RS12655 0.81 -0.4 2,297,028 + SGL_RS12655 0.86 -0.4 2,297,100 - SGL_RS12655 0.89 -0.2 2,297,113 - SGL_RS12655 0.90 -0.3 2,297,164 - -0.8 2,297,203 - +0.0 2,297,338 - -0.6 2,297,408 + -0.8 2,297,409 + -1.9 2,297,635 + SGL_RS12660 0.22 -3.0 2,297,635 + SGL_RS12660 0.22 -1.4 2,297,643 - SGL_RS12660 0.23 -1.9 2,297,672 - SGL_RS12660 0.25 -2.1 2,297,737 + SGL_RS12660 0.31 -2.4 2,297,885 + SGL_RS12660 0.43 -1.1 2,297,885 + SGL_RS12660 0.43 -1.1 2,297,893 - SGL_RS12660 0.44 -0.7 2,297,972 - SGL_RS12660 0.50 -1.9 2,298,047 + SGL_RS12660 0.56 +0.4 2,298,054 + SGL_RS12660 0.57 -2.1 2,298,087 - SGL_RS12660 0.60 -2.7 2,298,103 + SGL_RS12660 0.61 -1.6 2,298,172 - SGL_RS12660 0.67 -1.1 2,298,205 + SGL_RS12660 0.70 -1.9 2,298,213 - SGL_RS12660 0.70 -1.9 2,298,264 + SGL_RS12660 0.74 -3.0 2,298,380 + SGL_RS12660 0.84 -2.4 2,298,380 + SGL_RS12660 0.84 -1.6 2,298,380 + SGL_RS12660 0.84 -1.1 2,298,388 - SGL_RS12660 0.85 -2.2 2,298,388 - SGL_RS12660 0.85 -1.2 2,298,567 + -0.7 2,298,776 + SGL_RS12665 0.33 -0.4 2,298,784 - SGL_RS12665 0.35 +0.2 2,298,819 - SGL_RS12665 0.42 -0.5 2,298,819 - SGL_RS12665 0.42 -0.4 2,298,856 - SGL_RS12665 0.49 +0.0 2,298,857 + SGL_RS12665 0.49 -0.1 2,298,876 - SGL_RS12665 0.53 +0.2 2,298,925 + SGL_RS12665 0.62 +0.4 2,299,031 - SGL_RS12665 0.83 +0.0 2,299,070 - +0.4 2,299,103 - -0.3 2,299,120 + -0.1 2,299,247 - +0.7
Or see this region's nucleotide sequence