Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12565 and SGL_RS12570 are separated by 90 nucleotides SGL_RS12570 and SGL_RS12575 are separated by 260 nucleotides SGL_RS12575 and SGL_RS19930 are separated by 224 nucleotides SGL_RS19930 and SGL_RS12585 are separated by 67 nucleotides
SGL_RS12565: SGL_RS12565 - MEKHLA domain-containing protein, at 2,278,921 to 2,279,406
_RS12565
SGL_RS12570: SGL_RS12570 - pentapeptide repeat-containing protein, at 2,279,497 to 2,280,006
_RS12570
SGL_RS12575: SGL_RS12575 - riboflavin synthase, at 2,280,267 to 2,280,947
_RS12575
SGL_RS19930: SGL_RS19930 - hypothetical protein, at 2,281,172 to 2,281,441
_RS19930
SGL_RS12585: SGL_RS12585 - ABC transporter permease subunit, at 2,281,509 to 2,283,182
_RS12585
Position (kb)
2280
2281 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2279.287 kb on + strand, within SGL_RS12565 at 2279.872 kb on + strand, within SGL_RS12570 at 2279.896 kb on - strand, within SGL_RS12570 at 2279.896 kb on - strand, within SGL_RS12570 at 2279.917 kb on + strand, within SGL_RS12570 at 2280.416 kb on - strand, within SGL_RS12575 at 2280.715 kb on + strand, within SGL_RS12575 at 2280.715 kb on + strand, within SGL_RS12575 at 2280.723 kb on - strand, within SGL_RS12575 at 2280.734 kb on - strand, within SGL_RS12575 at 2280.791 kb on + strand, within SGL_RS12575 at 2280.799 kb on - strand, within SGL_RS12575 at 2280.836 kb on + strand, within SGL_RS12575 at 2280.966 kb on - strand at 2281.039 kb on + strand at 2281.149 kb on - strand at 2281.156 kb on - strand at 2281.337 kb on + strand, within SGL_RS19930 at 2281.503 kb on - strand at 2281.548 kb on - strand at 2281.661 kb on + strand at 2281.699 kb on + strand, within SGL_RS12585 at 2281.840 kb on - strand, within SGL_RS12585 at 2281.850 kb on + strand, within SGL_RS12585 at 2281.887 kb on - strand, within SGL_RS12585 at 2281.931 kb on + strand, within SGL_RS12585
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,279,287 + SGL_RS12565 0.75 -0.0 2,279,872 + SGL_RS12570 0.74 +0.1 2,279,896 - SGL_RS12570 0.78 +0.2 2,279,896 - SGL_RS12570 0.78 +0.1 2,279,917 + SGL_RS12570 0.82 +0.9 2,280,416 - SGL_RS12575 0.22 -3.8 2,280,715 + SGL_RS12575 0.66 -2.2 2,280,715 + SGL_RS12575 0.66 -0.9 2,280,723 - SGL_RS12575 0.67 -0.9 2,280,734 - SGL_RS12575 0.69 -3.6 2,280,791 + SGL_RS12575 0.77 -2.5 2,280,799 - SGL_RS12575 0.78 -1.9 2,280,836 + SGL_RS12575 0.84 -0.1 2,280,966 - +0.2 2,281,039 + +0.3 2,281,149 - +0.0 2,281,156 - +0.9 2,281,337 + SGL_RS19930 0.61 -0.8 2,281,503 - +0.9 2,281,548 - -1.9 2,281,661 + -1.5 2,281,699 + SGL_RS12585 0.11 -2.5 2,281,840 - SGL_RS12585 0.20 -2.5 2,281,850 + SGL_RS12585 0.20 -3.6 2,281,887 - SGL_RS12585 0.23 -1.9 2,281,931 + SGL_RS12585 0.25 -1.9
Or see this region's nucleotide sequence