Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12225 and SGL_RS12230 are separated by 99 nucleotides SGL_RS12230 and SGL_RS12235 are separated by 210 nucleotides SGL_RS12235 and SGL_RS12240 are separated by 82 nucleotides
SGL_RS12225: SGL_RS12225 - ATP-dependent Clp protease proteolytic subunit, at 2,205,774 to 2,206,451
_RS12225
SGL_RS12230: SGL_RS12230 - ATP-dependent Clp protease proteolytic subunit, at 2,206,551 to 2,207,159
_RS12230
SGL_RS12235: SGL_RS12235 - aminopeptidase P N-terminal domain-containing protein, at 2,207,370 to 2,208,695
_RS12235
SGL_RS12240: SGL_RS12240 - S-methyl-5'-thioadenosine phosphorylase, at 2,208,778 to 2,209,758
_RS12240
Position (kb)
2207
2208
2209 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2206.379 kb on - strand, within SGL_RS12225 at 2207.025 kb on + strand, within SGL_RS12230 at 2207.290 kb on - strand at 2207.358 kb on - strand at 2207.470 kb on - strand at 2207.540 kb on + strand, within SGL_RS12235 at 2208.128 kb on + strand, within SGL_RS12235 at 2208.215 kb on - strand, within SGL_RS12235 at 2208.407 kb on + strand, within SGL_RS12235 at 2208.609 kb on + strand at 2208.783 kb on + strand at 2208.791 kb on + strand at 2209.000 kb on - strand, within SGL_RS12240 at 2209.008 kb on - strand, within SGL_RS12240 at 2209.216 kb on + strand, within SGL_RS12240 at 2209.363 kb on + strand, within SGL_RS12240 at 2209.366 kb on - strand, within SGL_RS12240 at 2209.464 kb on + strand, within SGL_RS12240 at 2209.550 kb on - strand, within SGL_RS12240
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,206,379 - SGL_RS12225 0.89 -2.2 2,207,025 + SGL_RS12230 0.78 -1.9 2,207,290 - -1.3 2,207,358 - +0.1 2,207,470 - -1.5 2,207,540 + SGL_RS12235 0.13 -4.1 2,208,128 + SGL_RS12235 0.57 -3.6 2,208,215 - SGL_RS12235 0.64 -4.7 2,208,407 + SGL_RS12235 0.78 -3.7 2,208,609 + -3.9 2,208,783 + -0.2 2,208,791 + +0.1 2,209,000 - SGL_RS12240 0.23 +0.4 2,209,008 - SGL_RS12240 0.23 +0.2 2,209,216 + SGL_RS12240 0.45 +0.4 2,209,363 + SGL_RS12240 0.60 +0.4 2,209,366 - SGL_RS12240 0.60 +0.2 2,209,464 + SGL_RS12240 0.70 -0.4 2,209,550 - SGL_RS12240 0.79 -0.4
Or see this region's nucleotide sequence