Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12020 and SGL_RS12025 are separated by 99 nucleotides SGL_RS12025 and SGL_RS12030 overlap by 4 nucleotides
SGL_RS12020: SGL_RS12020 - NAD(P)H-binding protein, at 2,148,253 to 2,149,233
_RS12020
SGL_RS12025: SGL_RS12025 - NAD(+) kinase, at 2,149,333 to 2,150,250
_RS12025
SGL_RS12030: SGL_RS12030 - extracellular solute-binding protein, at 2,150,247 to 2,151,401
_RS12030
Position (kb)
2149
2150
2151 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2148.762 kb on + strand, within SGL_RS12020 at 2148.823 kb on + strand, within SGL_RS12020 at 2148.823 kb on + strand, within SGL_RS12020 at 2148.823 kb on + strand, within SGL_RS12020 at 2148.831 kb on - strand, within SGL_RS12020 at 2148.831 kb on - strand, within SGL_RS12020 at 2148.841 kb on - strand, within SGL_RS12020 at 2148.851 kb on + strand, within SGL_RS12020 at 2148.859 kb on - strand, within SGL_RS12020 at 2149.055 kb on - strand, within SGL_RS12020 at 2149.091 kb on - strand, within SGL_RS12020 at 2149.166 kb on - strand at 2149.333 kb on + strand at 2149.421 kb on + strand at 2149.429 kb on - strand, within SGL_RS12025 at 2149.542 kb on - strand, within SGL_RS12025 at 2149.566 kb on + strand, within SGL_RS12025 at 2149.576 kb on - strand, within SGL_RS12025 at 2149.596 kb on - strand, within SGL_RS12025 at 2149.615 kb on - strand, within SGL_RS12025 at 2149.641 kb on - strand, within SGL_RS12025 at 2149.669 kb on + strand, within SGL_RS12025 at 2149.803 kb on - strand, within SGL_RS12025 at 2150.074 kb on - strand, within SGL_RS12025 at 2150.090 kb on + strand, within SGL_RS12025 at 2150.108 kb on - strand, within SGL_RS12025 at 2150.332 kb on - strand at 2150.332 kb on - strand at 2150.332 kb on - strand at 2150.339 kb on + strand at 2150.347 kb on - strand at 2150.379 kb on - strand, within SGL_RS12030 at 2150.527 kb on + strand, within SGL_RS12030 at 2150.528 kb on - strand, within SGL_RS12030 at 2150.547 kb on - strand, within SGL_RS12030 at 2150.658 kb on - strand, within SGL_RS12030 at 2150.694 kb on + strand, within SGL_RS12030 at 2150.749 kb on - strand, within SGL_RS12030 at 2150.863 kb on + strand, within SGL_RS12030 at 2150.953 kb on - strand, within SGL_RS12030 at 2151.119 kb on + strand, within SGL_RS12030 at 2151.139 kb on - strand, within SGL_RS12030 at 2151.216 kb on - strand, within SGL_RS12030
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,148,762 + SGL_RS12020 0.52 +0.2 2,148,823 + SGL_RS12020 0.58 -0.3 2,148,823 + SGL_RS12020 0.58 -0.2 2,148,823 + SGL_RS12020 0.58 -0.4 2,148,831 - SGL_RS12020 0.59 -0.3 2,148,831 - SGL_RS12020 0.59 +0.6 2,148,841 - SGL_RS12020 0.60 -0.1 2,148,851 + SGL_RS12020 0.61 -0.4 2,148,859 - SGL_RS12020 0.62 -0.1 2,149,055 - SGL_RS12020 0.82 +0.3 2,149,091 - SGL_RS12020 0.85 -0.3 2,149,166 - -0.2 2,149,333 + -1.4 2,149,421 + +1.3 2,149,429 - SGL_RS12025 0.10 -2.2 2,149,542 - SGL_RS12025 0.23 -1.0 2,149,566 + SGL_RS12025 0.25 -1.5 2,149,576 - SGL_RS12025 0.26 -1.6 2,149,596 - SGL_RS12025 0.29 -1.9 2,149,615 - SGL_RS12025 0.31 -0.8 2,149,641 - SGL_RS12025 0.34 -1.1 2,149,669 + SGL_RS12025 0.37 -0.9 2,149,803 - SGL_RS12025 0.51 -1.8 2,150,074 - SGL_RS12025 0.81 -1.3 2,150,090 + SGL_RS12025 0.82 -1.7 2,150,108 - SGL_RS12025 0.84 -1.2 2,150,332 - -3.9 2,150,332 - -2.1 2,150,332 - -3.6 2,150,339 + -4.6 2,150,347 - -4.1 2,150,379 - SGL_RS12030 0.11 -3.9 2,150,527 + SGL_RS12030 0.24 -3.5 2,150,528 - SGL_RS12030 0.24 -3.1 2,150,547 - SGL_RS12030 0.26 -4.4 2,150,658 - SGL_RS12030 0.36 -2.9 2,150,694 + SGL_RS12030 0.39 -3.7 2,150,749 - SGL_RS12030 0.43 -2.5 2,150,863 + SGL_RS12030 0.53 -3.1 2,150,953 - SGL_RS12030 0.61 -3.1 2,151,119 + SGL_RS12030 0.75 -3.3 2,151,139 - SGL_RS12030 0.77 -2.2 2,151,216 - SGL_RS12030 0.84 -3.2
Or see this region's nucleotide sequence