Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11695 and SGL_RS11700 are separated by 143 nucleotides SGL_RS11700 and SGL_RS11705 are separated by 226 nucleotides SGL_RS11705 and SGL_RS18930 are separated by 111 nucleotides
SGL_RS11695: SGL_RS11695 - hypothetical protein, at 2,080,885 to 2,082,720
_RS11695
SGL_RS11700: SGL_RS11700 - 2OG-Fe(II) oxygenase, at 2,082,864 to 2,083,916
_RS11700
SGL_RS11705: SGL_RS11705 - DUF2281 domain-containing protein, at 2,084,143 to 2,084,370
_RS11705
SGL_RS18930: SGL_RS18930 - PIN domain-containing protein, at 2,084,482 to 2,084,760
_RS18930
Position (kb)
2082
2083
2084 Strain fitness (log2 ratio)
-1
0
1 at 2081.997 kb on + strand, within SGL_RS11695 at 2082.509 kb on + strand, within SGL_RS11695 at 2082.533 kb on + strand, within SGL_RS11695 at 2082.535 kb on - strand, within SGL_RS11695 at 2082.545 kb on + strand at 2082.613 kb on - strand at 2083.075 kb on + strand, within SGL_RS11700 at 2083.477 kb on + strand, within SGL_RS11700 at 2083.934 kb on + strand at 2084.038 kb on + strand at 2084.070 kb on + strand at 2084.073 kb on - strand at 2084.719 kb on + strand, within SGL_RS18930
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,081,997 + SGL_RS11695 0.61 +0.0 2,082,509 + SGL_RS11695 0.88 +0.2 2,082,533 + SGL_RS11695 0.90 +0.2 2,082,535 - SGL_RS11695 0.90 -0.2 2,082,545 + +0.4 2,082,613 - -0.1 2,083,075 + SGL_RS11700 0.20 +0.6 2,083,477 + SGL_RS11700 0.58 +0.1 2,083,934 + -0.2 2,084,038 + -0.4 2,084,070 + -0.3 2,084,073 - -0.0 2,084,719 + SGL_RS18930 0.85 +0.5
Or see this region's nucleotide sequence