Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11235 and SGL_RS11240 are separated by 577 nucleotides SGL_RS11240 and SGL_RS11245 are separated by 34 nucleotides SGL_RS11245 and SGL_RS11250 are separated by 32 nucleotides
SGL_RS11235: SGL_RS11235 - hybrid sensor histidine kinase/response regulator, at 1,980,365 to 1,981,471
_RS11235
SGL_RS11240: SGL_RS11240 - photosystem I reaction center subunit IV, at 1,982,049 to 1,982,273
_RS11240
SGL_RS11245: SGL_RS11245 - DNA-formamidopyrimidine glycosylase, at 1,982,308 to 1,983,171
_RS11245
SGL_RS11250: SGL_RS11250 - NUDIX domain-containing protein, at 1,983,204 to 1,983,932
_RS11250
Position (kb)
1982
1983 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1981.131 kb on + strand, within SGL_RS11235 at 1981.144 kb on - strand, within SGL_RS11235 at 1981.206 kb on + strand, within SGL_RS11235 at 1981.264 kb on - strand, within SGL_RS11235 at 1981.358 kb on - strand, within SGL_RS11235 at 1981.407 kb on - strand at 1981.417 kb on - strand at 1981.635 kb on + strand at 1981.794 kb on - strand at 1981.877 kb on + strand at 1981.926 kb on - strand at 1982.067 kb on + strand at 1982.214 kb on - strand, within SGL_RS11240 at 1982.368 kb on - strand at 1982.553 kb on + strand, within SGL_RS11245 at 1982.597 kb on + strand, within SGL_RS11245 at 1982.617 kb on + strand, within SGL_RS11245 at 1982.625 kb on - strand, within SGL_RS11245 at 1982.649 kb on - strand, within SGL_RS11245 at 1982.731 kb on - strand, within SGL_RS11245 at 1982.930 kb on + strand, within SGL_RS11245 at 1982.956 kb on - strand, within SGL_RS11245 at 1982.998 kb on - strand, within SGL_RS11245 at 1983.062 kb on - strand, within SGL_RS11245 at 1983.089 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 1,981,131 + SGL_RS11235 0.69 -2.7 1,981,144 - SGL_RS11235 0.70 -1.8 1,981,206 + SGL_RS11235 0.76 -1.8 1,981,264 - SGL_RS11235 0.81 -1.8 1,981,358 - SGL_RS11235 0.90 -2.0 1,981,407 - -3.2 1,981,417 - -2.5 1,981,635 + -0.5 1,981,794 - +0.2 1,981,877 + -0.3 1,981,926 - -0.6 1,982,067 + -0.7 1,982,214 - SGL_RS11240 0.73 -1.1 1,982,368 - -0.3 1,982,553 + SGL_RS11245 0.28 -0.3 1,982,597 + SGL_RS11245 0.33 -0.1 1,982,617 + SGL_RS11245 0.36 -0.2 1,982,625 - SGL_RS11245 0.37 -0.3 1,982,649 - SGL_RS11245 0.39 +0.3 1,982,731 - SGL_RS11245 0.49 -0.2 1,982,930 + SGL_RS11245 0.72 -0.1 1,982,956 - SGL_RS11245 0.75 +0.1 1,982,998 - SGL_RS11245 0.80 +0.2 1,983,062 - SGL_RS11245 0.87 -0.5 1,983,089 + -0.2
Or see this region's nucleotide sequence