Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11025 and SGL_RS11030 are separated by 16 nucleotides SGL_RS11030 and SGL_RS11035 are separated by 76 nucleotides SGL_RS11035 and SGL_RS11040 are separated by 118 nucleotides SGL_RS11040 and SGL_RS11045 are separated by 202 nucleotides
SGL_RS11025: SGL_RS11025 - oxidoreductase, at 1,935,912 to 1,937,069
_RS11025
SGL_RS11030: SGL_RS11030 - DUF433 domain-containing protein, at 1,937,086 to 1,937,397
_RS11030
SGL_RS11035: SGL_RS11035 - LapA family protein, at 1,937,474 to 1,938,310
_RS11035
SGL_RS11040: SGL_RS11040 - TMEM165/GDT1 family protein, at 1,938,429 to 1,938,782
_RS11040
SGL_RS11045: SGL_RS11045 - glutathione peroxidase, at 1,938,985 to 1,939,494
_RS11045
Position (kb)
1937
1938
1939 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1936.621 kb on - strand, within SGL_RS11025 at 1936.655 kb on - strand, within SGL_RS11025 at 1936.725 kb on - strand, within SGL_RS11025 at 1936.815 kb on + strand, within SGL_RS11025 at 1937.474 kb on + strand at 1937.641 kb on + strand, within SGL_RS11035 at 1937.902 kb on - strand, within SGL_RS11035 at 1937.978 kb on + strand, within SGL_RS11035 at 1938.415 kb on + strand at 1938.572 kb on + strand, within SGL_RS11040 at 1938.595 kb on - strand, within SGL_RS11040 at 1938.647 kb on - strand, within SGL_RS11040 at 1938.659 kb on + strand, within SGL_RS11040 at 1939.124 kb on + strand, within SGL_RS11045 at 1939.174 kb on - strand, within SGL_RS11045
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 1,936,621 - SGL_RS11025 0.61 -3.5 1,936,655 - SGL_RS11025 0.64 -1.8 1,936,725 - SGL_RS11025 0.70 -2.3 1,936,815 + SGL_RS11025 0.78 -1.3 1,937,474 + -0.2 1,937,641 + SGL_RS11035 0.20 -2.9 1,937,902 - SGL_RS11035 0.51 -1.7 1,937,978 + SGL_RS11035 0.60 -0.5 1,938,415 + -0.1 1,938,572 + SGL_RS11040 0.40 +0.5 1,938,595 - SGL_RS11040 0.47 +0.7 1,938,647 - SGL_RS11040 0.62 +0.2 1,938,659 + SGL_RS11040 0.65 +0.6 1,939,124 + SGL_RS11045 0.27 +0.3 1,939,174 - SGL_RS11045 0.37 +0.5
Or see this region's nucleotide sequence