Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10355 and SGL_RS10360 are separated by 145 nucleotides SGL_RS10360 and SGL_RS10365 are separated by 69 nucleotides SGL_RS10365 and SGL_RS10370 overlap by 97 nucleotides
SGL_RS10355: SGL_RS10355 - ABC transporter ATP-binding protein, at 1,797,552 to 1,798,658
_RS10355
SGL_RS10360: SGL_RS10360 - hypothetical protein, at 1,798,804 to 1,799,538
_RS10360
SGL_RS10365: SGL_RS10365 - CbiQ family ECF transporter T component, at 1,799,608 to 1,800,483
_RS10365
SGL_RS10370: SGL_RS10370 - anthranilate synthase component I, at 1,800,387 to 1,801,844
_RS10370
Position (kb) 1798
1799
1800 Strain fitness (log2 ratio) -2
-1
0
1 at 1798.834 kb on + strand at 1798.861 kb on - strand at 1798.901 kb on - strand, within SGL_RS10360 at 1798.969 kb on + strand, within SGL_RS10360 at 1799.061 kb on + strand, within SGL_RS10360 at 1799.074 kb on - strand, within SGL_RS10360 at 1799.351 kb on - strand, within SGL_RS10360 at 1799.423 kb on - strand, within SGL_RS10360 at 1799.476 kb on + strand at 1799.481 kb on + strand at 1799.491 kb on - strand at 1799.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 1,798,834 + -0.3 1,798,861 - -1.9 1,798,901 - SGL_RS10360 0.13 -2.0 1,798,969 + SGL_RS10360 0.22 -0.3 1,799,061 + SGL_RS10360 0.35 -0.2 1,799,074 - SGL_RS10360 0.37 -0.9 1,799,351 - SGL_RS10360 0.74 -1.0 1,799,423 - SGL_RS10360 0.84 -1.3 1,799,476 + -0.9 1,799,481 + -0.3 1,799,491 - -2.1 1,799,613 - -1.2
Or see this region's nucleotide sequence