Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09510 and SGL_RS09515 overlap by 4 nucleotides SGL_RS09515 and SGL_RS09520 are separated by 13 nucleotides SGL_RS09520 and SGL_RS09525 overlap by 11 nucleotides SGL_RS09525 and SGL_RS09530 are separated by 30 nucleotides
SGL_RS09510: SGL_RS09510 - beta-3-deoxy-D-manno-oct-2-ulosonic acid transferase, at 1,628,045 to 1,629,607
_RS09510
SGL_RS09515: SGL_RS09515 - glycosyltransferase family 2 protein, at 1,629,604 to 1,630,434
_RS09515
SGL_RS09520: SGL_RS09520 - DUF115 domain-containing protein, at 1,630,448 to 1,631,005
_RS09520
SGL_RS09525: SGL_RS09525 - hypothetical protein, at 1,630,995 to 1,631,240
_RS09525
SGL_RS09530: SGL_RS09530 - glycosyltransferase, at 1,631,271 to 1,632,530
_RS09530
Position (kb) 1630
1631
1632 Strain fitness (log2 ratio) -1
0
1 at 1629.486 kb on + strand at 1629.828 kb on - strand, within SGL_RS09515 at 1630.927 kb on + strand, within SGL_RS09520 at 1630.934 kb on - strand, within SGL_RS09520 at 1631.764 kb on + strand, within SGL_RS09530 at 1631.964 kb on + strand, within SGL_RS09530
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 1,629,486 + +0.2 1,629,828 - SGL_RS09515 0.27 +0.2 1,630,927 + SGL_RS09520 0.86 +0.3 1,630,934 - SGL_RS09520 0.87 +1.1 1,631,764 + SGL_RS09530 0.39 +1.2 1,631,964 + SGL_RS09530 0.55 +1.2
Or see this region's nucleotide sequence