Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08825 and SGL_RS08830 are separated by 167 nucleotides SGL_RS08830 and SGL_RS08835 are separated by 20 nucleotides SGL_RS08835 and SGL_RS08840 are separated by 422 nucleotides
SGL_RS08825: SGL_RS08825 - IS5 family transposase, at 1,483,391 to 1,484,044
_RS08825
SGL_RS08830: SGL_RS08830 - circadian clock KaiB family protein, at 1,484,212 to 1,484,538
_RS08830
SGL_RS08835: SGL_RS08835 - circadian clock protein KaiC, at 1,484,559 to 1,486,265
_RS08835
SGL_RS08840: SGL_RS08840 - DUF4230 domain-containing protein, at 1,486,688 to 1,487,410
_RS08840
Position (kb) 1484
1485
1486
1487 Strain fitness (log2 ratio) -1
0
1 at 1483.702 kb on - strand, within SGL_RS08825 at 1483.840 kb on - strand, within SGL_RS08825 at 1484.597 kb on + strand at 1484.755 kb on - strand, within SGL_RS08835 at 1484.762 kb on - strand, within SGL_RS08835 at 1485.711 kb on + strand, within SGL_RS08835 at 1485.892 kb on + strand, within SGL_RS08835 at 1486.105 kb on - strand at 1486.181 kb on - strand at 1486.412 kb on - strand at 1486.494 kb on + strand at 1486.521 kb on - strand at 1486.774 kb on - strand, within SGL_RS08840 at 1486.774 kb on - strand, within SGL_RS08840 at 1486.844 kb on + strand, within SGL_RS08840 at 1486.847 kb on - strand, within SGL_RS08840 at 1486.942 kb on - strand, within SGL_RS08840 at 1486.976 kb on - strand, within SGL_RS08840 at 1487.194 kb on - strand, within SGL_RS08840 at 1487.194 kb on - strand, within SGL_RS08840
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 1,483,702 - SGL_RS08825 0.48 -0.5 1,483,840 - SGL_RS08825 0.69 +0.0 1,484,597 + -1.7 1,484,755 - SGL_RS08835 0.11 -0.1 1,484,762 - SGL_RS08835 0.12 +0.0 1,485,711 + SGL_RS08835 0.67 +0.1 1,485,892 + SGL_RS08835 0.78 -0.4 1,486,105 - -0.0 1,486,181 - -0.2 1,486,412 - +0.1 1,486,494 + +0.1 1,486,521 - -0.3 1,486,774 - SGL_RS08840 0.12 -0.1 1,486,774 - SGL_RS08840 0.12 +0.1 1,486,844 + SGL_RS08840 0.22 -0.3 1,486,847 - SGL_RS08840 0.22 -0.1 1,486,942 - SGL_RS08840 0.35 -0.2 1,486,976 - SGL_RS08840 0.40 +0.1 1,487,194 - SGL_RS08840 0.70 +0.2 1,487,194 - SGL_RS08840 0.70 +0.4
Or see this region's nucleotide sequence