Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08265 and SGL_RS08270 overlap by 22 nucleotides SGL_RS08270 and SGL_RS08275 are separated by 46 nucleotides SGL_RS08275 and SGL_RS19845 are separated by 199 nucleotides SGL_RS19845 and SGL_RS19850 are separated by 4 nucleotides SGL_RS19850 and SGL_RS19455 are separated by 1 nucleotides
SGL_RS08265: SGL_RS08265 - hypothetical protein, at 1,363,265 to 1,363,858
_RS08265
SGL_RS08270: SGL_RS08270 - orotidine-5'-phosphate decarboxylase, at 1,363,837 to 1,364,532
_RS08270
SGL_RS08275: SGL_RS08275 - tetratricopeptide repeat protein, at 1,364,579 to 1,365,448
_RS08275
SGL_RS19845: SGL_RS19845 - hypothetical protein, at 1,365,648 to 1,365,803
_RS19845
SGL_RS19850: SGL_RS19850 - hypothetical protein, at 1,365,808 to 1,366,215
_RS19850
SGL_RS19455: SGL_RS19455 - hypothetical protein, at 1,366,217 to 1,366,378
_RS19455
Position (kb)
1364
1365
1366 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1363.680 kb on - strand, within SGL_RS08265 at 1363.697 kb on + strand, within SGL_RS08265 at 1363.759 kb on - strand, within SGL_RS08265 at 1364.882 kb on + strand, within SGL_RS08275 at 1365.119 kb on + strand, within SGL_RS08275 at 1365.125 kb on + strand, within SGL_RS08275 at 1365.139 kb on - strand, within SGL_RS08275 at 1365.151 kb on + strand, within SGL_RS08275 at 1365.156 kb on + strand, within SGL_RS08275 at 1365.159 kb on - strand, within SGL_RS08275 at 1365.223 kb on - strand, within SGL_RS08275 at 1365.354 kb on + strand, within SGL_RS08275 at 1365.362 kb on - strand at 1365.417 kb on - strand at 1365.493 kb on + strand at 1365.553 kb on + strand at 1365.553 kb on + strand at 1365.561 kb on - strand at 1365.561 kb on - strand at 1365.584 kb on + strand at 1365.638 kb on + strand at 1365.797 kb on + strand at 1365.804 kb on + strand at 1365.805 kb on - strand at 1365.812 kb on - strand at 1365.829 kb on + strand at 1365.837 kb on - strand at 1365.901 kb on - strand, within SGL_RS19850 at 1365.970 kb on - strand, within SGL_RS19850 at 1365.970 kb on - strand, within SGL_RS19850 at 1365.996 kb on - strand, within SGL_RS19850 at 1366.013 kb on + strand, within SGL_RS19850 at 1366.013 kb on + strand, within SGL_RS19850 at 1366.037 kb on - strand, within SGL_RS19850 at 1366.058 kb on - strand, within SGL_RS19850 at 1366.087 kb on - strand, within SGL_RS19850 at 1366.152 kb on + strand, within SGL_RS19850 at 1366.171 kb on + strand, within SGL_RS19850
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 1,363,680 - SGL_RS08265 0.70 -0.2 1,363,697 + SGL_RS08265 0.73 +0.1 1,363,759 - SGL_RS08265 0.83 +0.2 1,364,882 + SGL_RS08275 0.35 -2.4 1,365,119 + SGL_RS08275 0.62 -3.7 1,365,125 + SGL_RS08275 0.63 -1.3 1,365,139 - SGL_RS08275 0.64 -3.3 1,365,151 + SGL_RS08275 0.66 -3.0 1,365,156 + SGL_RS08275 0.66 -3.0 1,365,159 - SGL_RS08275 0.67 -1.3 1,365,223 - SGL_RS08275 0.74 -1.6 1,365,354 + SGL_RS08275 0.89 -3.1 1,365,362 - -1.8 1,365,417 - -1.0 1,365,493 + -1.7 1,365,553 + -2.5 1,365,553 + -1.3 1,365,561 - -1.6 1,365,561 - -1.3 1,365,584 + +0.2 1,365,638 + +0.4 1,365,797 + -0.7 1,365,804 + +0.5 1,365,805 - +0.1 1,365,812 - +1.0 1,365,829 + -0.1 1,365,837 - +0.2 1,365,901 - SGL_RS19850 0.23 -0.7 1,365,970 - SGL_RS19850 0.40 +0.1 1,365,970 - SGL_RS19850 0.40 -0.2 1,365,996 - SGL_RS19850 0.46 +0.2 1,366,013 + SGL_RS19850 0.50 -0.8 1,366,013 + SGL_RS19850 0.50 -0.1 1,366,037 - SGL_RS19850 0.56 +0.7 1,366,058 - SGL_RS19850 0.61 -0.0 1,366,087 - SGL_RS19850 0.68 +0.3 1,366,152 + SGL_RS19850 0.84 -0.3 1,366,171 + SGL_RS19850 0.89 +0.2
Or see this region's nucleotide sequence