Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06135 and SGL_RS06140 are separated by 362 nucleotides SGL_RS06140 and SGL_RS06145 are separated by 412 nucleotides
SGL_RS06135: SGL_RS06135 - 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD, at 909,603 to 910,994
_RS06135
SGL_RS06140: SGL_RS06140 - threonine ammonia-lyase, biosynthetic, at 911,357 to 912,883
_RS06140
SGL_RS06145: SGL_RS06145 - cell division protein SepF, at 913,296 to 913,856
_RS06145
Position (kb)
911
912
913 Strain fitness (log2 ratio)
-2
-1
0
1 at 910.364 kb on + strand, within SGL_RS06135 at 910.364 kb on + strand, within SGL_RS06135 at 910.372 kb on - strand, within SGL_RS06135 at 910.401 kb on + strand, within SGL_RS06135 at 910.521 kb on - strand, within SGL_RS06135 at 910.565 kb on - strand, within SGL_RS06135 at 910.595 kb on - strand, within SGL_RS06135 at 910.605 kb on + strand, within SGL_RS06135 at 910.607 kb on + strand, within SGL_RS06135 at 910.615 kb on - strand, within SGL_RS06135 at 910.623 kb on - strand, within SGL_RS06135 at 910.658 kb on - strand, within SGL_RS06135 at 910.747 kb on - strand, within SGL_RS06135 at 910.758 kb on + strand, within SGL_RS06135 at 910.774 kb on - strand, within SGL_RS06135 at 910.856 kb on - strand at 911.037 kb on + strand at 911.203 kb on - strand at 911.272 kb on + strand at 911.272 kb on + strand at 911.277 kb on + strand at 911.280 kb on - strand at 911.285 kb on - strand at 911.403 kb on - strand at 911.597 kb on - strand, within SGL_RS06140 at 911.597 kb on - strand, within SGL_RS06140 at 911.605 kb on + strand, within SGL_RS06140 at 911.666 kb on - strand, within SGL_RS06140 at 911.735 kb on + strand, within SGL_RS06140 at 911.735 kb on + strand, within SGL_RS06140 at 911.863 kb on - strand, within SGL_RS06140 at 911.905 kb on - strand, within SGL_RS06140 at 911.922 kb on + strand, within SGL_RS06140 at 911.930 kb on - strand, within SGL_RS06140 at 912.048 kb on + strand, within SGL_RS06140 at 912.050 kb on + strand, within SGL_RS06140 at 912.156 kb on + strand, within SGL_RS06140 at 912.176 kb on + strand, within SGL_RS06140 at 912.330 kb on - strand, within SGL_RS06140 at 912.334 kb on + strand, within SGL_RS06140 at 912.406 kb on - strand, within SGL_RS06140 at 912.479 kb on + strand, within SGL_RS06140 at 912.524 kb on - strand, within SGL_RS06140 at 912.598 kb on - strand, within SGL_RS06140 at 912.696 kb on + strand, within SGL_RS06140 at 912.709 kb on - strand, within SGL_RS06140 at 912.812 kb on - strand at 912.821 kb on + strand at 912.948 kb on + strand at 912.948 kb on + strand at 912.977 kb on - strand at 913.803 kb on - strand at 913.869 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 910,364 + SGL_RS06135 0.55 +0.1 910,364 + SGL_RS06135 0.55 -0.2 910,372 - SGL_RS06135 0.55 +0.1 910,401 + SGL_RS06135 0.57 +0.0 910,521 - SGL_RS06135 0.66 -0.1 910,565 - SGL_RS06135 0.69 +0.9 910,595 - SGL_RS06135 0.71 +0.2 910,605 + SGL_RS06135 0.72 +0.2 910,607 + SGL_RS06135 0.72 +0.1 910,615 - SGL_RS06135 0.73 +1.2 910,623 - SGL_RS06135 0.73 -0.4 910,658 - SGL_RS06135 0.76 -0.1 910,747 - SGL_RS06135 0.82 -0.1 910,758 + SGL_RS06135 0.83 +0.2 910,774 - SGL_RS06135 0.84 +0.3 910,856 - -0.1 911,037 + +0.1 911,203 - +0.2 911,272 + -0.1 911,272 + +0.1 911,277 + -0.1 911,280 - +0.2 911,285 - +0.4 911,403 - -0.9 911,597 - SGL_RS06140 0.16 -1.4 911,597 - SGL_RS06140 0.16 -0.7 911,605 + SGL_RS06140 0.16 -0.1 911,666 - SGL_RS06140 0.20 -1.1 911,735 + SGL_RS06140 0.25 -1.1 911,735 + SGL_RS06140 0.25 -0.3 911,863 - SGL_RS06140 0.33 -0.4 911,905 - SGL_RS06140 0.36 -1.0 911,922 + SGL_RS06140 0.37 -1.1 911,930 - SGL_RS06140 0.38 -0.8 912,048 + SGL_RS06140 0.45 -0.6 912,050 + SGL_RS06140 0.45 -0.9 912,156 + SGL_RS06140 0.52 -0.7 912,176 + SGL_RS06140 0.54 -1.3 912,330 - SGL_RS06140 0.64 -2.5 912,334 + SGL_RS06140 0.64 -1.3 912,406 - SGL_RS06140 0.69 -2.2 912,479 + SGL_RS06140 0.73 -2.0 912,524 - SGL_RS06140 0.76 -1.0 912,598 - SGL_RS06140 0.81 -1.3 912,696 + SGL_RS06140 0.88 -1.1 912,709 - SGL_RS06140 0.89 -1.3 912,812 - -1.3 912,821 + -1.1 912,948 + -0.1 912,948 + +0.2 912,977 - +0.1 913,803 - +0.1 913,869 + -0.6
Or see this region's nucleotide sequence