Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05970 and SGL_RS05975 are separated by 153 nucleotides SGL_RS05975 and SGL_RS05980 are separated by 28 nucleotides SGL_RS05980 and SGL_RS05985 are separated by 27 nucleotides
SGL_RS05970: SGL_RS05970 - DUF533 domain-containing protein, at 874,693 to 875,361
_RS05970
SGL_RS05975: SGL_RS05975 - DUF3531 family protein, at 875,515 to 875,979
_RS05975
SGL_RS05980: SGL_RS05980 - hypothetical protein, at 876,008 to 877,297
_RS05980
SGL_RS05985: SGL_RS05985 - DNA-processing protein DprA, at 877,325 to 878,521
_RS05985
Position (kb)
876
877
878 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 875.130 kb on - strand, within SGL_RS05970 at 875.201 kb on - strand, within SGL_RS05970 at 875.216 kb on + strand, within SGL_RS05970 at 875.341 kb on - strand at 875.603 kb on + strand, within SGL_RS05975 at 875.756 kb on + strand, within SGL_RS05975 at 875.756 kb on + strand, within SGL_RS05975 at 876.126 kb on - strand at 876.133 kb on + strand at 876.139 kb on + strand, within SGL_RS05980 at 876.392 kb on + strand, within SGL_RS05980 at 876.404 kb on + strand, within SGL_RS05980 at 876.446 kb on + strand, within SGL_RS05980 at 876.563 kb on - strand, within SGL_RS05980 at 876.684 kb on - strand, within SGL_RS05980 at 876.725 kb on + strand, within SGL_RS05980 at 876.804 kb on + strand, within SGL_RS05980 at 876.913 kb on + strand, within SGL_RS05980 at 876.964 kb on + strand, within SGL_RS05980 at 877.040 kb on - strand, within SGL_RS05980 at 877.169 kb on + strand at 877.202 kb on - strand at 877.366 kb on + strand at 877.388 kb on - strand at 877.411 kb on - strand at 877.628 kb on + strand, within SGL_RS05985 at 877.655 kb on - strand, within SGL_RS05985 at 877.805 kb on - strand, within SGL_RS05985 at 878.008 kb on + strand, within SGL_RS05985 at 878.011 kb on - strand, within SGL_RS05985 at 878.016 kb on - strand, within SGL_RS05985 at 878.038 kb on + strand, within SGL_RS05985 at 878.059 kb on - strand, within SGL_RS05985 at 878.059 kb on - strand, within SGL_RS05985 at 878.093 kb on + strand, within SGL_RS05985 at 878.101 kb on - strand, within SGL_RS05985 at 878.101 kb on - strand, within SGL_RS05985
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 875,130 - SGL_RS05970 0.65 -1.5 875,201 - SGL_RS05970 0.76 -0.2 875,216 + SGL_RS05970 0.78 +0.6 875,341 - +0.4 875,603 + SGL_RS05975 0.19 +0.1 875,756 + SGL_RS05975 0.52 -0.1 875,756 + SGL_RS05975 0.52 +0.2 876,126 - -3.2 876,133 + -1.7 876,139 + SGL_RS05980 0.10 -1.8 876,392 + SGL_RS05980 0.30 +2.4 876,404 + SGL_RS05980 0.31 -3.9 876,446 + SGL_RS05980 0.34 -1.0 876,563 - SGL_RS05980 0.43 -1.5 876,684 - SGL_RS05980 0.52 -1.8 876,725 + SGL_RS05980 0.56 +0.7 876,804 + SGL_RS05980 0.62 -1.5 876,913 + SGL_RS05980 0.70 -0.9 876,964 + SGL_RS05980 0.74 -2.2 877,040 - SGL_RS05980 0.80 -0.7 877,169 + -0.8 877,202 - -0.7 877,366 + +0.2 877,388 - +0.1 877,411 - -0.2 877,628 + SGL_RS05985 0.25 +0.2 877,655 - SGL_RS05985 0.28 -0.0 877,805 - SGL_RS05985 0.40 -0.2 878,008 + SGL_RS05985 0.57 -0.2 878,011 - SGL_RS05985 0.57 -0.0 878,016 - SGL_RS05985 0.58 -0.2 878,038 + SGL_RS05985 0.60 -0.0 878,059 - SGL_RS05985 0.61 -0.0 878,059 - SGL_RS05985 0.61 +0.0 878,093 + SGL_RS05985 0.64 -0.0 878,101 - SGL_RS05985 0.65 +0.0 878,101 - SGL_RS05985 0.65 +0.2
Or see this region's nucleotide sequence