Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05400 and SGL_RS05405 are separated by 555 nucleotides SGL_RS05405 and SGL_RS05410 are separated by 48 nucleotides SGL_RS05410 and SGL_RS05415 overlap by 50 nucleotides
SGL_RS05400: SGL_RS05400 - Crp/Fnr family transcriptional regulator, at 754,877 to 755,497
_RS05400
SGL_RS05405: SGL_RS05405 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 756,053 to 757,195
_RS05405
SGL_RS05410: SGL_RS05410 - type II secretion system protein, at 757,244 to 757,720
_RS05410
SGL_RS05415: SGL_RS05415 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 757,671 to 758,753
_RS05415
Position (kb)
756
757
758 Strain fitness (log2 ratio)
-1
0
1
2 at 755.149 kb on - strand, within SGL_RS05400 at 755.273 kb on - strand, within SGL_RS05400 at 755.790 kb on - strand at 755.969 kb on + strand at 756.186 kb on + strand, within SGL_RS05405 at 756.194 kb on - strand, within SGL_RS05405 at 756.198 kb on + strand, within SGL_RS05405 at 756.324 kb on + strand, within SGL_RS05405 at 756.386 kb on - strand, within SGL_RS05405 at 756.415 kb on - strand, within SGL_RS05405 at 756.570 kb on - strand, within SGL_RS05405 at 756.727 kb on - strand, within SGL_RS05405 at 756.733 kb on + strand, within SGL_RS05405 at 756.802 kb on + strand, within SGL_RS05405 at 757.022 kb on + strand, within SGL_RS05405 at 757.022 kb on + strand, within SGL_RS05405 at 757.065 kb on - strand, within SGL_RS05405 at 757.387 kb on - strand, within SGL_RS05410 at 757.504 kb on - strand, within SGL_RS05410 at 758.029 kb on + strand, within SGL_RS05415
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 755,149 - SGL_RS05400 0.44 +1.3 755,273 - SGL_RS05400 0.64 +1.1 755,790 - +1.9 755,969 + +0.8 756,186 + SGL_RS05405 0.12 +1.4 756,194 - SGL_RS05405 0.12 +1.5 756,198 + SGL_RS05405 0.13 +1.4 756,324 + SGL_RS05405 0.24 +1.5 756,386 - SGL_RS05405 0.29 +1.3 756,415 - SGL_RS05405 0.32 +1.3 756,570 - SGL_RS05405 0.45 +1.3 756,727 - SGL_RS05405 0.59 +1.5 756,733 + SGL_RS05405 0.59 +1.3 756,802 + SGL_RS05405 0.66 +1.3 757,022 + SGL_RS05405 0.85 +1.3 757,022 + SGL_RS05405 0.85 +1.0 757,065 - SGL_RS05405 0.89 +2.0 757,387 - SGL_RS05410 0.30 +1.0 757,504 - SGL_RS05410 0.55 +1.2 758,029 + SGL_RS05415 0.33 +1.5
Or see this region's nucleotide sequence