Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05225 and SGL_RS05230 are separated by 231 nucleotides SGL_RS05230 and SGL_RS05235 are separated by 146 nucleotides SGL_RS05235 and SGL_RS05240 are separated by 45 nucleotides SGL_RS05240 and SGL_RS05245 are separated by 114 nucleotides
SGL_RS05225: SGL_RS05225 - serine--tRNA ligase, at 722,570 to 723,862
_RS05225
SGL_RS05230: SGL_RS05230 - phycobilisome linker polypeptide, at 724,094 to 724,345
_RS05230
SGL_RS05235: SGL_RS05235 - phycobilisome linker polypeptide, at 724,492 to 725,367
_RS05235
SGL_RS05240: SGL_RS05240 - phycobilisome linker polypeptide, at 725,413 to 726,234
_RS05240
SGL_RS05245: SGL_RS05245 - phycocyanin subunit alpha, at 726,349 to 726,837
_RS05245
Position (kb) 724
725
726 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 724.531 kb on - strand at 724.563 kb on - strand at 724.713 kb on - strand, within SGL_RS05235 at 724.799 kb on + strand, within SGL_RS05235 at 724.950 kb on - strand, within SGL_RS05235 at 725.233 kb on + strand, within SGL_RS05235 at 725.554 kb on - strand, within SGL_RS05240 at 726.100 kb on + strand, within SGL_RS05240 at 726.190 kb on - strand at 726.200 kb on - strand at 726.321 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 724,531 - +0.5 724,563 - -0.6 724,713 - SGL_RS05235 0.25 -1.5 724,799 + SGL_RS05235 0.35 -1.8 724,950 - SGL_RS05235 0.52 -1.8 725,233 + SGL_RS05235 0.85 -3.0 725,554 - SGL_RS05240 0.17 -1.2 726,100 + SGL_RS05240 0.84 -1.7 726,190 - -1.4 726,200 - -0.5 726,321 + -2.3
Or see this region's nucleotide sequence