Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04730 and SGL_RS04735 are separated by 23 nucleotides SGL_RS04735 and SGL_RS04740 overlap by 4 nucleotides SGL_RS04740 and SGL_RS04745 are separated by 264 nucleotides
SGL_RS04730: SGL_RS04730 - N-acetylmuramoyl-L-alanine amidase, at 613,801 to 614,817
_RS04730
SGL_RS04735: SGL_RS04735 - hypothetical protein, at 614,841 to 615,269
_RS04735
SGL_RS04740: SGL_RS04740 - DUF1036 domain-containing protein, at 615,266 to 615,703
_RS04740
SGL_RS04745: SGL_RS04745 - STAS domain-containing protein, at 615,968 to 616,324
_RS04745
Position (kb) 614
615
616 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 613.878 kb on - strand at 613.883 kb on - strand at 613.884 kb on + strand at 613.884 kb on + strand at 613.959 kb on + strand, within SGL_RS04730 at 613.970 kb on + strand, within SGL_RS04730 at 614.073 kb on + strand, within SGL_RS04730 at 614.113 kb on + strand, within SGL_RS04730 at 614.145 kb on + strand, within SGL_RS04730 at 614.153 kb on - strand, within SGL_RS04730 at 614.176 kb on - strand, within SGL_RS04730 at 614.216 kb on - strand, within SGL_RS04730 at 614.296 kb on - strand, within SGL_RS04730 at 614.364 kb on + strand, within SGL_RS04730 at 614.377 kb on + strand, within SGL_RS04730 at 614.551 kb on - strand, within SGL_RS04730 at 614.604 kb on - strand, within SGL_RS04730 at 614.623 kb on - strand, within SGL_RS04730 at 614.627 kb on + strand, within SGL_RS04730 at 614.706 kb on + strand, within SGL_RS04730 at 614.890 kb on - strand, within SGL_RS04735 at 614.945 kb on - strand, within SGL_RS04735 at 614.989 kb on - strand, within SGL_RS04735 at 615.030 kb on - strand, within SGL_RS04735 at 615.108 kb on - strand, within SGL_RS04735 at 615.210 kb on - strand, within SGL_RS04735 at 615.337 kb on - strand, within SGL_RS04740 at 615.484 kb on - strand, within SGL_RS04740 at 615.484 kb on - strand, within SGL_RS04740 at 615.497 kb on + strand, within SGL_RS04740 at 615.504 kb on - strand, within SGL_RS04740 at 615.506 kb on - strand, within SGL_RS04740 at 615.506 kb on - strand, within SGL_RS04740 at 615.508 kb on + strand, within SGL_RS04740 at 615.566 kb on + strand, within SGL_RS04740 at 615.574 kb on - strand, within SGL_RS04740 at 615.711 kb on + strand at 615.748 kb on + strand at 615.748 kb on + strand at 615.781 kb on + strand at 615.789 kb on - strand at 615.789 kb on - strand at 616.013 kb on + strand, within SGL_RS04745 at 616.183 kb on + strand, within SGL_RS04745
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 613,878 - +0.3 613,883 - +0.0 613,884 + -0.2 613,884 + +0.0 613,959 + SGL_RS04730 0.16 +0.1 613,970 + SGL_RS04730 0.17 -0.2 614,073 + SGL_RS04730 0.27 -0.3 614,113 + SGL_RS04730 0.31 -0.6 614,145 + SGL_RS04730 0.34 +0.3 614,153 - SGL_RS04730 0.35 -0.1 614,176 - SGL_RS04730 0.37 -0.3 614,216 - SGL_RS04730 0.41 +0.6 614,296 - SGL_RS04730 0.49 -0.3 614,364 + SGL_RS04730 0.55 -0.2 614,377 + SGL_RS04730 0.57 -0.9 614,551 - SGL_RS04730 0.74 +0.7 614,604 - SGL_RS04730 0.79 -1.8 614,623 - SGL_RS04730 0.81 +0.0 614,627 + SGL_RS04730 0.81 +0.0 614,706 + SGL_RS04730 0.89 +0.4 614,890 - SGL_RS04735 0.11 -0.8 614,945 - SGL_RS04735 0.24 -3.6 614,989 - SGL_RS04735 0.34 -0.8 615,030 - SGL_RS04735 0.44 -4.1 615,108 - SGL_RS04735 0.62 -2.0 615,210 - SGL_RS04735 0.86 -2.0 615,337 - SGL_RS04740 0.16 -1.1 615,484 - SGL_RS04740 0.50 +0.1 615,484 - SGL_RS04740 0.50 +0.5 615,497 + SGL_RS04740 0.53 +0.3 615,504 - SGL_RS04740 0.54 +0.2 615,506 - SGL_RS04740 0.55 +0.3 615,506 - SGL_RS04740 0.55 -0.0 615,508 + SGL_RS04740 0.55 -0.2 615,566 + SGL_RS04740 0.68 -0.7 615,574 - SGL_RS04740 0.70 -0.0 615,711 + +0.1 615,748 + -0.6 615,748 + +0.2 615,781 + -0.2 615,789 - -0.1 615,789 - +1.0 616,013 + SGL_RS04745 0.13 -0.6 616,183 + SGL_RS04745 0.60 +0.7
Or see this region's nucleotide sequence