Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04715 and SGL_RS04720 are separated by 569 nucleotides SGL_RS04720 and SGL_RS19335 are separated by 91 nucleotides SGL_RS19335 and SGL_RS04725 are separated by 19 nucleotides SGL_RS04725 and SGL_RS04730 are separated by 57 nucleotides
SGL_RS04715: SGL_RS04715 - hypothetical protein, at 610,053 to 610,487
_RS04715
SGL_RS04720: SGL_RS04720 - iron uptake porin, at 611,057 to 612,832
_RS04720
SGL_RS19335: SGL_RS19335 - hypothetical protein, at 612,924 to 613,082
_RS19335
SGL_RS04725: SGL_RS04725 - response regulator transcription factor, at 613,102 to 613,743
_RS04725
SGL_RS04730: SGL_RS04730 - N-acetylmuramoyl-L-alanine amidase, at 613,801 to 614,817
_RS04730
Position (kb) 611
612
613 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 610.114 kb on - strand, within SGL_RS04715 at 610.255 kb on + strand, within SGL_RS04715 at 610.256 kb on - strand, within SGL_RS04715 at 610.461 kb on + strand at 610.468 kb on + strand at 610.558 kb on + strand at 610.810 kb on - strand at 611.107 kb on - strand at 611.143 kb on + strand at 611.239 kb on + strand, within SGL_RS04720 at 611.248 kb on + strand, within SGL_RS04720 at 611.678 kb on + strand, within SGL_RS04720 at 611.758 kb on + strand, within SGL_RS04720 at 612.071 kb on - strand, within SGL_RS04720 at 612.141 kb on + strand, within SGL_RS04720 at 612.252 kb on + strand, within SGL_RS04720 at 612.255 kb on - strand, within SGL_RS04720 at 612.260 kb on - strand, within SGL_RS04720 at 612.811 kb on - strand at 612.836 kb on - strand at 613.161 kb on - strand at 613.218 kb on - strand, within SGL_RS04725 at 613.312 kb on + strand, within SGL_RS04725 at 613.559 kb on - strand, within SGL_RS04725 at 613.659 kb on - strand, within SGL_RS04725 at 613.713 kb on - strand at 613.713 kb on - strand at 613.770 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 610,114 - SGL_RS04715 0.14 +0.3 610,255 + SGL_RS04715 0.46 +0.0 610,256 - SGL_RS04715 0.47 +0.1 610,461 + +0.1 610,468 + -0.0 610,558 + +0.4 610,810 - -0.1 611,107 - -1.0 611,143 + -4.1 611,239 + SGL_RS04720 0.10 -1.8 611,248 + SGL_RS04720 0.11 -1.5 611,678 + SGL_RS04720 0.35 -3.3 611,758 + SGL_RS04720 0.39 -3.3 612,071 - SGL_RS04720 0.57 -2.5 612,141 + SGL_RS04720 0.61 -2.5 612,252 + SGL_RS04720 0.67 -2.5 612,255 - SGL_RS04720 0.67 -1.0 612,260 - SGL_RS04720 0.68 -3.0 612,811 - -4.1 612,836 - -0.8 613,161 - -0.3 613,218 - SGL_RS04725 0.18 +0.7 613,312 + SGL_RS04725 0.33 +0.2 613,559 - SGL_RS04725 0.71 -0.0 613,659 - SGL_RS04725 0.87 +0.9 613,713 - +0.9 613,713 - +0.8 613,770 + +0.1
Or see this region's nucleotide sequence