Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04565 and SGL_RS04570 are separated by 12 nucleotides SGL_RS04570 and SGL_RS04575 are separated by 11 nucleotides SGL_RS04575 and SGL_RS04580 are separated by 63 nucleotides
SGL_RS04565: SGL_RS04565 - hypothetical protein, at 574,592 to 575,464
_RS04565
SGL_RS04570: SGL_RS04570 - Uma2 family endonuclease, at 575,477 to 576,127
_RS04570
SGL_RS04575: SGL_RS04575 - hypothetical protein, at 576,139 to 576,870
_RS04575
SGL_RS04580: SGL_RS04580 - DNA topoisomerase (ATP-hydrolyzing) subunit A, at 576,934 to 579,696
_RS04580
Position (kb) 576
577 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 575.396 kb on + strand at 575.732 kb on - strand, within SGL_RS04570 at 575.967 kb on - strand, within SGL_RS04570 at 576.156 kb on + strand at 576.164 kb on - strand at 576.170 kb on + strand at 576.170 kb on + strand at 576.178 kb on - strand at 576.291 kb on - strand, within SGL_RS04575 at 576.293 kb on + strand, within SGL_RS04575 at 576.421 kb on - strand, within SGL_RS04575 at 576.512 kb on + strand, within SGL_RS04575 at 576.584 kb on - strand, within SGL_RS04575 at 576.595 kb on + strand, within SGL_RS04575 at 576.637 kb on - strand, within SGL_RS04575 at 576.639 kb on + strand, within SGL_RS04575 at 576.658 kb on - strand, within SGL_RS04575 at 576.731 kb on + strand, within SGL_RS04575 at 576.872 kb on - strand at 576.932 kb on - strand at 577.015 kb on - strand at 577.033 kb on + strand at 577.046 kb on - strand at 577.092 kb on - strand at 577.209 kb on - strand at 577.342 kb on + strand, within SGL_RS04580 at 577.712 kb on + strand, within SGL_RS04580 at 577.829 kb on + strand, within SGL_RS04580
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 575,396 + +0.2 575,732 - SGL_RS04570 0.39 -0.1 575,967 - SGL_RS04570 0.75 -0.1 576,156 + +1.8 576,164 - +2.2 576,170 + +1.9 576,170 + +1.5 576,178 - +2.0 576,291 - SGL_RS04575 0.21 +1.7 576,293 + SGL_RS04575 0.21 +1.3 576,421 - SGL_RS04575 0.39 +2.0 576,512 + SGL_RS04575 0.51 +1.6 576,584 - SGL_RS04575 0.61 +2.2 576,595 + SGL_RS04575 0.62 +2.1 576,637 - SGL_RS04575 0.68 +1.5 576,639 + SGL_RS04575 0.68 +1.7 576,658 - SGL_RS04575 0.71 +2.0 576,731 + SGL_RS04575 0.81 +1.5 576,872 - +1.3 576,932 - +0.5 577,015 - -0.5 577,033 + -1.7 577,046 - -1.5 577,092 - -3.3 577,209 - -0.8 577,342 + SGL_RS04580 0.15 -1.0 577,712 + SGL_RS04580 0.28 -1.0 577,829 + SGL_RS04580 0.32 -0.3
Or see this region's nucleotide sequence