Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04225 and SGL_RS19430 are separated by 61 nucleotides SGL_RS19430 and SGL_RS04230 are separated by 2 nucleotides SGL_RS04230 and SGL_RS04235 are separated by 81 nucleotides SGL_RS04235 and SGL_RS04240 are separated by 75 nucleotides
SGL_RS04225: SGL_RS04225 - sensor domain-containing diguanylate cyclase, at 496,450 to 497,844
_RS04225
SGL_RS19430: SGL_RS19430 - hypothetical protein, at 497,906 to 498,073
_RS19430
SGL_RS04230: SGL_RS04230 - exonuclease subunit SbcC, at 498,076 to 501,096
_RS04230
SGL_RS04235: SGL_RS04235 - pentapeptide repeat-containing protein, at 501,178 to 501,966
_RS04235
SGL_RS04240: SGL_RS04240 - 50S ribosomal protein L32, at 502,042 to 502,215
_RS04240
Position (kb) 498
499
500
501
502 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 497.144 kb on + strand, within SGL_RS04225 at 497.612 kb on + strand, within SGL_RS04225 at 497.640 kb on + strand, within SGL_RS04225 at 497.650 kb on - strand, within SGL_RS04225 at 498.031 kb on - strand, within SGL_RS19430 at 498.154 kb on + strand at 498.168 kb on + strand at 498.168 kb on + strand at 498.377 kb on + strand at 498.410 kb on + strand, within SGL_RS04230 at 499.092 kb on + strand, within SGL_RS04230 at 499.284 kb on + strand, within SGL_RS04230 at 499.284 kb on + strand, within SGL_RS04230 at 499.296 kb on + strand, within SGL_RS04230 at 499.975 kb on - strand, within SGL_RS04230 at 500.789 kb on + strand, within SGL_RS04230 at 500.890 kb on + strand at 500.890 kb on + strand at 500.898 kb on - strand at 500.989 kb on + strand at 501.194 kb on - strand at 501.297 kb on - strand, within SGL_RS04235 at 501.660 kb on + strand, within SGL_RS04235 at 501.674 kb on + strand, within SGL_RS04235 at 501.676 kb on - strand, within SGL_RS04235 at 501.910 kb on - strand at 501.920 kb on - strand at 501.932 kb on - strand at 501.966 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 497,144 + SGL_RS04225 0.50 -0.7 497,612 + SGL_RS04225 0.83 +0.5 497,640 + SGL_RS04225 0.85 +0.1 497,650 - SGL_RS04225 0.86 +0.2 498,031 - SGL_RS19430 0.74 -2.8 498,154 + -1.1 498,168 + -1.4 498,168 + -3.8 498,377 + -2.3 498,410 + SGL_RS04230 0.11 -2.3 499,092 + SGL_RS04230 0.34 -2.8 499,284 + SGL_RS04230 0.40 -4.1 499,284 + SGL_RS04230 0.40 -2.1 499,296 + SGL_RS04230 0.40 -2.4 499,975 - SGL_RS04230 0.63 -1.6 500,789 + SGL_RS04230 0.90 -1.3 500,890 + -2.7 500,890 + -3.3 500,898 - -1.3 500,989 + -3.8 501,194 - +0.3 501,297 - SGL_RS04235 0.15 -0.1 501,660 + SGL_RS04235 0.61 -0.3 501,674 + SGL_RS04235 0.63 -0.2 501,676 - SGL_RS04235 0.63 +0.3 501,910 - +0.5 501,920 - -0.5 501,932 - +0.0 501,966 - +0.1
Or see this region's nucleotide sequence