Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS19425 and SGL_RS04080 are separated by 31 nucleotides SGL_RS04080 and SGL_RS04085 are separated by 94 nucleotides SGL_RS04085 and SGL_RS04090 are separated by 163 nucleotides SGL_RS04090 and SGL_RS04095 are separated by 139 nucleotides SGL_RS04095 and SGL_RS04100 are separated by 22 nucleotides
SGL_RS19425: SGL_RS19425 - hypothetical protein, at 465,560 to 465,718
_RS19425
SGL_RS04080: SGL_RS04080 - NnrU family protein, at 465,750 to 466,493
_RS04080
SGL_RS04085: SGL_RS04085 - YlqD family protein, at 466,588 to 467,037
_RS04085
SGL_RS04090: SGL_RS04090 - photosystem I reaction center subunit XII, at 467,201 to 467,296
_RS04090
SGL_RS04095: SGL_RS04095 - hypothetical protein, at 467,436 to 467,813
_RS04095
SGL_RS04100: SGL_RS04100 - UDP-3-O-acyl-N-acetylglucosamine deacetylase, at 467,836 to 468,666
_RS04100
Position (kb) 466
467
468 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 465.651 kb on + strand, within SGL_RS19425 at 465.689 kb on + strand, within SGL_RS19425 at 465.788 kb on + strand at 465.845 kb on + strand, within SGL_RS04080 at 465.875 kb on - strand, within SGL_RS04080 at 465.926 kb on + strand, within SGL_RS04080 at 466.026 kb on + strand, within SGL_RS04080 at 466.037 kb on + strand, within SGL_RS04080 at 466.045 kb on - strand, within SGL_RS04080 at 466.238 kb on - strand, within SGL_RS04080 at 466.411 kb on + strand, within SGL_RS04080 at 466.442 kb on - strand at 466.459 kb on + strand at 466.549 kb on - strand at 466.612 kb on - strand at 466.640 kb on - strand, within SGL_RS04085 at 466.683 kb on - strand, within SGL_RS04085 at 466.693 kb on - strand, within SGL_RS04085 at 466.738 kb on - strand, within SGL_RS04085 at 467.031 kb on + strand at 467.040 kb on - strand at 467.049 kb on - strand at 467.153 kb on + strand at 467.221 kb on + strand, within SGL_RS04090 at 467.633 kb on - strand, within SGL_RS04095 at 467.816 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 465,651 + SGL_RS19425 0.57 -0.1 465,689 + SGL_RS19425 0.81 +0.3 465,788 + -0.2 465,845 + SGL_RS04080 0.13 +0.6 465,875 - SGL_RS04080 0.17 +0.2 465,926 + SGL_RS04080 0.24 +0.7 466,026 + SGL_RS04080 0.37 +0.0 466,037 + SGL_RS04080 0.39 +0.1 466,045 - SGL_RS04080 0.40 +0.3 466,238 - SGL_RS04080 0.66 -0.8 466,411 + SGL_RS04080 0.89 +0.1 466,442 - -0.2 466,459 + -0.1 466,549 - -1.2 466,612 - -1.8 466,640 - SGL_RS04085 0.12 -1.5 466,683 - SGL_RS04085 0.21 -0.4 466,693 - SGL_RS04085 0.23 -4.4 466,738 - SGL_RS04085 0.33 -1.1 467,031 + +0.7 467,040 - -0.5 467,049 - +0.2 467,153 + -0.6 467,221 + SGL_RS04090 0.21 -0.4 467,633 - SGL_RS04095 0.52 -2.8 467,816 - +0.3
Or see this region's nucleotide sequence