Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03865 and SGL_RS03870 are separated by 237 nucleotides SGL_RS03870 and SGL_RS18680 are separated by 33 nucleotides SGL_RS18680 and SGL_RS19780 overlap by 91 nucleotides
SGL_RS03865: SGL_RS03865 - calcium/sodium antiporter, at 419,161 to 420,264
_RS03865
SGL_RS03870: SGL_RS03870 - histidinol dehydrogenase, at 420,502 to 421,731
_RS03870
SGL_RS18680: SGL_RS18680 - IS630 family transposase, at 421,765 to 422,343
_RS18680
SGL_RS19780: SGL_RS19780 - IS630 transposase-related protein, at 422,253 to 422,612
_RS19780
Position (kb) 420
421
422 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 419.894 kb on + strand, within SGL_RS03865 at 420.203 kb on + strand at 420.203 kb on + strand at 420.203 kb on + strand at 420.203 kb on + strand at 420.211 kb on - strand at 420.211 kb on - strand at 420.211 kb on - strand at 420.211 kb on - strand at 420.306 kb on - strand at 420.313 kb on + strand at 420.321 kb on - strand at 420.353 kb on + strand at 420.376 kb on + strand at 420.407 kb on + strand at 420.430 kb on - strand at 420.442 kb on + strand at 420.464 kb on + strand at 420.466 kb on + strand at 420.474 kb on - strand at 420.559 kb on - strand at 420.653 kb on - strand, within SGL_RS03870 at 420.720 kb on + strand, within SGL_RS03870 at 420.728 kb on - strand, within SGL_RS03870 at 420.738 kb on - strand, within SGL_RS03870 at 420.865 kb on - strand, within SGL_RS03870 at 420.902 kb on - strand, within SGL_RS03870 at 420.921 kb on + strand, within SGL_RS03870 at 420.998 kb on + strand, within SGL_RS03870 at 421.026 kb on + strand, within SGL_RS03870 at 421.082 kb on - strand, within SGL_RS03870 at 421.228 kb on + strand, within SGL_RS03870 at 421.251 kb on - strand, within SGL_RS03870 at 421.251 kb on - strand, within SGL_RS03870 at 421.355 kb on + strand, within SGL_RS03870 at 421.443 kb on + strand, within SGL_RS03870 at 421.445 kb on - strand, within SGL_RS03870 at 421.492 kb on + strand, within SGL_RS03870 at 421.550 kb on + strand, within SGL_RS03870 at 421.705 kb on - strand at 421.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 419,894 + SGL_RS03865 0.66 +0.1 420,203 + +0.3 420,203 + +0.4 420,203 + +0.3 420,203 + -0.3 420,211 - -0.4 420,211 - +0.3 420,211 - -0.4 420,211 - +0.0 420,306 - -0.2 420,313 + +0.2 420,321 - +0.1 420,353 + +0.7 420,376 + +0.4 420,407 + +0.4 420,430 - +0.5 420,442 + -0.9 420,464 + -1.3 420,466 + -1.4 420,474 - -1.8 420,559 - -1.0 420,653 - SGL_RS03870 0.12 -1.9 420,720 + SGL_RS03870 0.18 -1.6 420,728 - SGL_RS03870 0.18 -1.9 420,738 - SGL_RS03870 0.19 -2.0 420,865 - SGL_RS03870 0.30 -1.8 420,902 - SGL_RS03870 0.33 -1.8 420,921 + SGL_RS03870 0.34 -2.1 420,998 + SGL_RS03870 0.40 -1.8 421,026 + SGL_RS03870 0.43 -1.6 421,082 - SGL_RS03870 0.47 -1.7 421,228 + SGL_RS03870 0.59 -1.9 421,251 - SGL_RS03870 0.61 -2.0 421,251 - SGL_RS03870 0.61 -4.5 421,355 + SGL_RS03870 0.69 -1.8 421,443 + SGL_RS03870 0.77 -1.5 421,445 - SGL_RS03870 0.77 -2.4 421,492 + SGL_RS03870 0.80 -1.3 421,550 + SGL_RS03870 0.85 -2.1 421,705 - -1.4 421,710 - -0.1
Or see this region's nucleotide sequence