Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03830 and SGL_RS20115 are separated by 32 nucleotides SGL_RS20115 and SGL_RS03840 are separated by 168 nucleotides SGL_RS03840 and SGL_RS03845 are separated by 157 nucleotides SGL_RS03845 and SGL_RS03850 are separated by 110 nucleotides
SGL_RS03830: SGL_RS03830 - choice-of-anchor I family protein, at 410,315 to 414,544
_RS03830
SGL_RS20115: SGL_RS20115 - hypothetical protein, at 414,577 to 414,702
_RS20115
SGL_RS03840: SGL_RS03840 - adenylyl-sulfate kinase, at 414,871 to 415,404
_RS03840
SGL_RS03845: SGL_RS03845 - MotA/TolQ/ExbB proton channel family protein, at 415,562 to 416,245
_RS03845
SGL_RS03850: SGL_RS03850 - biopolymer transporter ExbD, at 416,356 to 417,165
_RS03850
Position (kb)
414
415
416 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 413.876 kb on - strand, within SGL_RS03830 at 414.095 kb on - strand, within SGL_RS03830 at 414.300 kb on + strand at 414.308 kb on - strand at 414.308 kb on - strand at 414.318 kb on + strand at 414.387 kb on + strand at 414.387 kb on + strand at 414.395 kb on - strand at 414.596 kb on - strand, within SGL_RS20115 at 414.598 kb on + strand, within SGL_RS20115 at 414.612 kb on - strand, within SGL_RS20115 at 414.798 kb on + strand at 414.877 kb on - strand at 414.884 kb on + strand at 414.968 kb on - strand, within SGL_RS03840 at 415.201 kb on - strand, within SGL_RS03840 at 415.323 kb on - strand, within SGL_RS03840 at 415.344 kb on + strand, within SGL_RS03840 at 415.418 kb on - strand at 415.418 kb on - strand at 415.726 kb on + strand, within SGL_RS03845 at 415.838 kb on + strand, within SGL_RS03845 at 415.910 kb on + strand, within SGL_RS03845 at 415.913 kb on - strand, within SGL_RS03845 at 415.939 kb on - strand, within SGL_RS03845 at 416.033 kb on + strand, within SGL_RS03845 at 416.038 kb on + strand, within SGL_RS03845 at 416.103 kb on + strand, within SGL_RS03845
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 413,876 - SGL_RS03830 0.84 -0.2 414,095 - SGL_RS03830 0.89 +0.3 414,300 + -0.2 414,308 - -0.6 414,308 - -0.1 414,318 + +0.1 414,387 + -0.1 414,387 + -0.3 414,395 - -0.2 414,596 - SGL_RS20115 0.15 +0.3 414,598 + SGL_RS20115 0.17 -0.1 414,612 - SGL_RS20115 0.28 +0.3 414,798 + +0.1 414,877 - -0.0 414,884 + -3.3 414,968 - SGL_RS03840 0.18 -4.1 415,201 - SGL_RS03840 0.62 -1.4 415,323 - SGL_RS03840 0.85 -1.7 415,344 + SGL_RS03840 0.89 -1.5 415,418 - -1.0 415,418 - +0.3 415,726 + SGL_RS03845 0.24 +1.4 415,838 + SGL_RS03845 0.40 +1.2 415,910 + SGL_RS03845 0.51 +2.7 415,913 - SGL_RS03845 0.51 +1.9 415,939 - SGL_RS03845 0.55 +1.5 416,033 + SGL_RS03845 0.69 +1.4 416,038 + SGL_RS03845 0.70 +1.4 416,103 + SGL_RS03845 0.79 +1.7
Or see this region's nucleotide sequence