Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03355 and SGL_RS03360 are separated by 89 nucleotides SGL_RS03360 and SGL_RS03365 are separated by 198 nucleotides SGL_RS03365 and SGL_RS03370 are separated by 52 nucleotides SGL_RS03370 and SGL_RS03375 are separated by 129 nucleotides
SGL_RS03355: SGL_RS03355 - hypothetical protein, at 317,984 to 318,571
_RS03355
SGL_RS03360: SGL_RS03360 - 50S ribosomal protein L28, at 318,661 to 318,897
_RS03360
SGL_RS03365: SGL_RS03365 - Ycf66 family protein, at 319,096 to 320,529
_RS03365
SGL_RS03370: SGL_RS03370 - tRNA-Pro, at 320,582 to 320,655
_RS03370
SGL_RS03375: SGL_RS03375 - hypothetical protein, at 320,785 to 321,447
_RS03375
Position (kb) 319
320
321 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 318.428 kb on + strand, within SGL_RS03355 at 318.436 kb on - strand, within SGL_RS03355 at 318.436 kb on - strand, within SGL_RS03355 at 318.438 kb on - strand, within SGL_RS03355 at 318.508 kb on + strand, within SGL_RS03355 at 318.833 kb on - strand, within SGL_RS03360 at 318.849 kb on - strand, within SGL_RS03360 at 318.924 kb on + strand at 319.009 kb on - strand at 319.932 kb on + strand, within SGL_RS03365 at 320.090 kb on - strand, within SGL_RS03365 at 320.147 kb on - strand, within SGL_RS03365 at 320.183 kb on - strand, within SGL_RS03365 at 320.450 kb on - strand at 320.511 kb on + strand at 320.703 kb on + strand at 320.909 kb on + strand, within SGL_RS03375 at 320.928 kb on + strand, within SGL_RS03375 at 321.116 kb on - strand, within SGL_RS03375 at 321.176 kb on + strand, within SGL_RS03375 at 321.176 kb on + strand, within SGL_RS03375 at 321.176 kb on + strand, within SGL_RS03375 at 321.240 kb on - strand, within SGL_RS03375 at 321.332 kb on + strand, within SGL_RS03375 at 321.351 kb on - strand, within SGL_RS03375 at 321.368 kb on + strand, within SGL_RS03375 at 321.387 kb on + strand at 321.388 kb on + strand at 321.505 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 318,428 + SGL_RS03355 0.76 -0.1 318,436 - SGL_RS03355 0.77 +0.0 318,436 - SGL_RS03355 0.77 +0.4 318,438 - SGL_RS03355 0.77 -0.1 318,508 + SGL_RS03355 0.89 -0.7 318,833 - SGL_RS03360 0.73 -0.2 318,849 - SGL_RS03360 0.79 +0.0 318,924 + -0.1 319,009 - +0.2 319,932 + SGL_RS03365 0.58 -2.9 320,090 - SGL_RS03365 0.69 -1.2 320,147 - SGL_RS03365 0.73 -2.9 320,183 - SGL_RS03365 0.76 -2.1 320,450 - -0.9 320,511 + -1.6 320,703 + +1.6 320,909 + SGL_RS03375 0.19 +0.5 320,928 + SGL_RS03375 0.22 -0.1 321,116 - SGL_RS03375 0.50 -0.1 321,176 + SGL_RS03375 0.59 +0.1 321,176 + SGL_RS03375 0.59 -0.0 321,176 + SGL_RS03375 0.59 -1.0 321,240 - SGL_RS03375 0.69 +0.1 321,332 + SGL_RS03375 0.83 +0.4 321,351 - SGL_RS03375 0.85 +0.1 321,368 + SGL_RS03375 0.88 +0.3 321,387 + +0.1 321,388 + +2.0 321,505 + +0.0
Or see this region's nucleotide sequence