Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03175 and SGL_RS03180 overlap by 50 nucleotides SGL_RS03180 and SGL_RS03185 are separated by 39 nucleotides SGL_RS03185 and SGL_RS03190 are separated by 18 nucleotides SGL_RS03190 and SGL_RS03195 are separated by 63 nucleotides
SGL_RS03175: SGL_RS03175 - hypothetical protein, at 278,494 to 279,561
_RS03175
SGL_RS03180: SGL_RS03180 - DUF3611 family protein, at 279,512 to 280,087
_RS03180
SGL_RS03185: SGL_RS03185 - biotin--[acetyl-CoA-carboxylase] ligase, at 280,127 to 280,951
_RS03185
SGL_RS03190: SGL_RS03190 - universal stress protein, at 280,970 to 281,443
_RS03190
SGL_RS03195: SGL_RS03195 - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE, at 281,507 to 282,223
_RS03195
Position (kb) 280
281 Strain fitness (log2 ratio) -2
-1
0
1 at 279.134 kb on - strand, within SGL_RS03175 at 279.282 kb on + strand, within SGL_RS03175 at 279.351 kb on - strand, within SGL_RS03175 at 279.392 kb on - strand, within SGL_RS03175 at 279.452 kb on + strand, within SGL_RS03175 at 279.489 kb on + strand at 279.690 kb on - strand, within SGL_RS03180 at 280.024 kb on + strand, within SGL_RS03180 at 280.029 kb on - strand, within SGL_RS03180 at 280.064 kb on - strand at 280.082 kb on + strand at 280.176 kb on - strand at 280.242 kb on + strand, within SGL_RS03185 at 280.350 kb on - strand, within SGL_RS03185 at 280.499 kb on - strand, within SGL_RS03185 at 280.579 kb on + strand, within SGL_RS03185 at 280.633 kb on - strand, within SGL_RS03185 at 280.644 kb on + strand, within SGL_RS03185 at 280.706 kb on + strand, within SGL_RS03185 at 280.840 kb on - strand, within SGL_RS03185 at 280.849 kb on - strand, within SGL_RS03185 at 280.875 kb on + strand at 280.876 kb on - strand at 280.926 kb on + strand at 280.934 kb on - strand at 280.976 kb on - strand at 280.976 kb on - strand at 280.987 kb on - strand at 280.992 kb on - strand at 280.992 kb on - strand at 281.019 kb on - strand, within SGL_RS03190 at 281.043 kb on - strand, within SGL_RS03190 at 281.321 kb on - strand, within SGL_RS03190 at 281.326 kb on - strand, within SGL_RS03190 at 281.326 kb on - strand, within SGL_RS03190 at 281.399 kb on + strand at 281.399 kb on - strand at 281.531 kb on + strand at 281.562 kb on + strand at 281.618 kb on - strand, within SGL_RS03195 at 281.643 kb on - strand, within SGL_RS03195 at 281.681 kb on - strand, within SGL_RS03195 at 281.691 kb on - strand, within SGL_RS03195 at 281.860 kb on - strand, within SGL_RS03195 at 281.873 kb on + strand, within SGL_RS03195
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 279,134 - SGL_RS03175 0.60 -0.4 279,282 + SGL_RS03175 0.74 -0.2 279,351 - SGL_RS03175 0.80 -1.0 279,392 - SGL_RS03175 0.84 -0.9 279,452 + SGL_RS03175 0.90 -0.4 279,489 + -0.1 279,690 - SGL_RS03180 0.31 -0.2 280,024 + SGL_RS03180 0.89 +0.1 280,029 - SGL_RS03180 0.90 -1.0 280,064 - -1.2 280,082 + -0.9 280,176 - +0.2 280,242 + SGL_RS03185 0.14 +0.3 280,350 - SGL_RS03185 0.27 -2.5 280,499 - SGL_RS03185 0.45 -0.3 280,579 + SGL_RS03185 0.55 -0.9 280,633 - SGL_RS03185 0.61 -1.9 280,644 + SGL_RS03185 0.63 -2.6 280,706 + SGL_RS03185 0.70 +0.7 280,840 - SGL_RS03185 0.86 -2.2 280,849 - SGL_RS03185 0.88 -0.9 280,875 + -2.5 280,876 - -0.9 280,926 + -0.3 280,934 - +0.0 280,976 - -0.0 280,976 - -0.2 280,987 - -0.6 280,992 - +0.2 280,992 - +0.1 281,019 - SGL_RS03190 0.10 -2.2 281,043 - SGL_RS03190 0.15 -2.4 281,321 - SGL_RS03190 0.74 +0.0 281,326 - SGL_RS03190 0.75 -0.7 281,326 - SGL_RS03190 0.75 -1.3 281,399 + +0.2 281,399 - -0.0 281,531 + -0.2 281,562 + -0.2 281,618 - SGL_RS03195 0.15 +0.1 281,643 - SGL_RS03195 0.19 -0.1 281,681 - SGL_RS03195 0.24 +0.3 281,691 - SGL_RS03195 0.26 +0.0 281,860 - SGL_RS03195 0.49 +0.6 281,873 + SGL_RS03195 0.51 +0.4
Or see this region's nucleotide sequence