Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02795 and SGL_RS19405 are separated by 118 nucleotides SGL_RS19405 and SGL_RS02800 are separated by 46 nucleotides SGL_RS02800 and SGL_RS02805 are separated by 80 nucleotides SGL_RS02805 and SGL_RS02810 are separated by 75 nucleotides
SGL_RS02795: SGL_RS02795 - flavin prenyltransferase UbiX, at 195,685 to 196,305
_RS02795
SGL_RS19405: SGL_RS19405 - hypothetical protein, at 196,424 to 196,564
_RS19405
SGL_RS02800: SGL_RS02800 - hypothetical protein, at 196,611 to 196,991
_RS02800
SGL_RS02805: SGL_RS02805 - universal stress protein, at 197,072 to 197,557
_RS02805
SGL_RS02810: SGL_RS02810 - EAL domain-containing protein, at 197,633 to 200,185
_RS02810
Position (kb) 197
198 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 196.230 kb on + strand, within SGL_RS02795 at 196.238 kb on - strand, within SGL_RS02795 at 196.334 kb on - strand at 196.357 kb on - strand at 196.448 kb on + strand, within SGL_RS19405 at 196.456 kb on - strand, within SGL_RS19405 at 196.607 kb on - strand at 196.763 kb on - strand, within SGL_RS02800 at 196.851 kb on + strand, within SGL_RS02800 at 196.882 kb on - strand, within SGL_RS02800 at 196.973 kb on + strand at 196.981 kb on - strand at 197.152 kb on + strand, within SGL_RS02805 at 197.152 kb on + strand, within SGL_RS02805 at 197.153 kb on + strand, within SGL_RS02805 at 197.155 kb on + strand, within SGL_RS02805 at 197.160 kb on - strand, within SGL_RS02805 at 197.276 kb on - strand, within SGL_RS02805 at 197.423 kb on + strand, within SGL_RS02805 at 197.423 kb on - strand, within SGL_RS02805 at 197.433 kb on - strand, within SGL_RS02805 at 197.445 kb on - strand, within SGL_RS02805 at 197.519 kb on + strand at 197.655 kb on + strand at 197.708 kb on + strand at 197.791 kb on + strand at 197.868 kb on + strand at 197.874 kb on - strand at 198.006 kb on + strand, within SGL_RS02810 at 198.235 kb on - strand, within SGL_RS02810 at 198.305 kb on + strand, within SGL_RS02810 at 198.340 kb on + strand, within SGL_RS02810 at 198.464 kb on - strand, within SGL_RS02810 at 198.523 kb on - strand, within SGL_RS02810 at 198.555 kb on + strand, within SGL_RS02810
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 196,230 + SGL_RS02795 0.88 -3.7 196,238 - SGL_RS02795 0.89 -3.8 196,334 - -0.6 196,357 - +0.1 196,448 + SGL_RS19405 0.17 +0.3 196,456 - SGL_RS19405 0.23 -0.2 196,607 - -0.5 196,763 - SGL_RS02800 0.40 -0.5 196,851 + SGL_RS02800 0.63 -0.4 196,882 - SGL_RS02800 0.71 -0.2 196,973 + -2.2 196,981 - -0.9 197,152 + SGL_RS02805 0.16 -3.0 197,152 + SGL_RS02805 0.16 -3.2 197,153 + SGL_RS02805 0.17 -3.8 197,155 + SGL_RS02805 0.17 -2.0 197,160 - SGL_RS02805 0.18 -2.9 197,276 - SGL_RS02805 0.42 -3.6 197,423 + SGL_RS02805 0.72 -3.4 197,423 - SGL_RS02805 0.72 -3.7 197,433 - SGL_RS02805 0.74 -2.0 197,445 - SGL_RS02805 0.77 -2.5 197,519 + -3.7 197,655 + -4.3 197,708 + -5.2 197,791 + -3.5 197,868 + -3.3 197,874 - -4.1 198,006 + SGL_RS02810 0.15 -3.4 198,235 - SGL_RS02810 0.24 -3.4 198,305 + SGL_RS02810 0.26 -4.7 198,340 + SGL_RS02810 0.28 -4.4 198,464 - SGL_RS02810 0.33 -2.8 198,523 - SGL_RS02810 0.35 -3.8 198,555 + SGL_RS02810 0.36 -4.2
Or see this region's nucleotide sequence