Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02525 and SGL_RS02530 are separated by 82 nucleotides SGL_RS02530 and SGL_RS02540 are separated by 231 nucleotides SGL_RS02540 and SGL_RS18650 are separated by 108 nucleotides SGL_RS18650 and SGL_RS02545 are separated by 148 nucleotides
SGL_RS02525: SGL_RS02525 - hypothetical protein, at 144,554 to 145,081
_RS02525
SGL_RS02530: SGL_RS02530 - MTH1187 family thiamine-binding protein, at 145,164 to 145,529
_RS02530
SGL_RS02540: SGL_RS02540 - universal stress protein, at 145,761 to 146,615
_RS02540
SGL_RS18650: SGL_RS18650 - photosystem II reaction center protein PsbM, at 146,724 to 146,831
_RS18650
SGL_RS02545: SGL_RS02545 - histone deacetylase, at 146,980 to 147,894
_RS02545
Position (kb) 145
146
147 Strain fitness (log2 ratio) -1
0
1 at 144.766 kb on + strand, within SGL_RS02525 at 144.821 kb on + strand, within SGL_RS02525 at 145.410 kb on - strand, within SGL_RS02530 at 145.499 kb on - strand at 145.564 kb on + strand at 145.585 kb on - strand at 145.596 kb on - strand at 145.659 kb on - strand at 145.665 kb on - strand at 145.671 kb on + strand at 145.672 kb on + strand at 145.805 kb on - strand at 145.814 kb on + strand at 146.011 kb on - strand, within SGL_RS02540 at 146.453 kb on + strand, within SGL_RS02540 at 146.461 kb on - strand, within SGL_RS02540 at 146.508 kb on + strand, within SGL_RS02540 at 146.553 kb on - strand at 146.691 kb on + strand at 146.812 kb on - strand, within SGL_RS18650 at 146.840 kb on - strand at 146.880 kb on - strand at 146.963 kb on + strand at 147.028 kb on - strand at 147.036 kb on + strand at 147.052 kb on - strand at 147.284 kb on + strand, within SGL_RS02545 at 147.301 kb on - strand, within SGL_RS02545 at 147.325 kb on + strand, within SGL_RS02545 at 147.339 kb on - strand, within SGL_RS02545 at 147.466 kb on + strand, within SGL_RS02545
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 144,766 + SGL_RS02525 0.40 -0.5 144,821 + SGL_RS02525 0.51 +0.0 145,410 - SGL_RS02530 0.67 +0.5 145,499 - -0.0 145,564 + -0.0 145,585 - +0.4 145,596 - -0.0 145,659 - -0.2 145,665 - -0.0 145,671 + -0.3 145,672 + -0.8 145,805 - -0.6 145,814 + -0.2 146,011 - SGL_RS02540 0.29 -1.3 146,453 + SGL_RS02540 0.81 -1.2 146,461 - SGL_RS02540 0.82 -1.0 146,508 + SGL_RS02540 0.87 -0.8 146,553 - -0.0 146,691 + -0.1 146,812 - SGL_RS18650 0.81 -0.2 146,840 - -0.3 146,880 - -0.3 146,963 + -0.4 147,028 - -0.6 147,036 + -0.9 147,052 - -0.5 147,284 + SGL_RS02545 0.33 -0.4 147,301 - SGL_RS02545 0.35 -0.4 147,325 + SGL_RS02545 0.38 -0.9 147,339 - SGL_RS02545 0.39 -1.0 147,466 + SGL_RS02545 0.53 -0.4
Or see this region's nucleotide sequence