Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02360 and SGL_RS02365 are separated by 130 nucleotides SGL_RS02365 and SGL_RS02370 are separated by 249 nucleotides SGL_RS02370 and SGL_RS02375 overlap by 4 nucleotides
SGL_RS02360: SGL_RS02360 - O-antigen ligase, at 112,860 to 114,188
_RS02360
SGL_RS02365: SGL_RS02365 - DUF3181 family protein, at 114,319 to 114,624
_RS02365
SGL_RS02370: SGL_RS02370 - DUF3119 family protein, at 114,874 to 115,272
_RS02370
SGL_RS02375: SGL_RS02375 - DUF3086 domain-containing protein, at 115,269 to 116,477
_RS02375
Position (kb) 114
115 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 113.380 kb on + strand, within SGL_RS02360 at 114.339 kb on - strand at 114.494 kb on - strand, within SGL_RS02365 at 114.713 kb on - strand at 114.788 kb on + strand at 114.856 kb on + strand at 114.856 kb on + strand at 114.884 kb on + strand at 114.978 kb on + strand, within SGL_RS02370 at 115.049 kb on + strand, within SGL_RS02370 at 115.415 kb on + strand, within SGL_RS02375 at 115.512 kb on + strand, within SGL_RS02375 at 115.586 kb on + strand, within SGL_RS02375 at 115.586 kb on + strand, within SGL_RS02375
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 113,380 + SGL_RS02360 0.39 -1.5 114,339 - +0.1 114,494 - SGL_RS02365 0.57 +0.1 114,713 - +0.4 114,788 + +0.5 114,856 + -0.5 114,856 + +0.7 114,884 + -0.2 114,978 + SGL_RS02370 0.26 -0.4 115,049 + SGL_RS02370 0.44 +0.1 115,415 + SGL_RS02375 0.12 -3.7 115,512 + SGL_RS02375 0.20 -3.1 115,586 + SGL_RS02375 0.26 -4.4 115,586 + SGL_RS02375 0.26 -2.7
Or see this region's nucleotide sequence