Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02205 and SGL_RS02210 are separated by 153 nucleotides SGL_RS02210 and SGL_RS02215 overlap by 41 nucleotides SGL_RS02215 and SGL_RS02220 are separated by 21 nucleotides SGL_RS02220 and SGL_RS02225 are separated by 137 nucleotides SGL_RS02225 and SGL_RS02230 are separated by 5 nucleotides
SGL_RS02205: SGL_RS02205 - thioredoxin family protein, at 77,494 to 77,826
_RS02205
SGL_RS02210: SGL_RS02210 - DUF2442 domain-containing protein, at 77,980 to 78,246
_RS02210
SGL_RS02215: SGL_RS02215 - DUF4160 domain-containing protein, at 78,206 to 78,445
_RS02215
SGL_RS02220: SGL_RS02220 - phosphoribosylformylglycinamidine synthase subunit PurL, at 78,467 to 80,800
_RS02220
SGL_RS02225: SGL_RS02225 - hypothetical protein, at 80,938 to 81,690
_RS02225
SGL_RS02230: SGL_RS02230 - NUDIX hydrolase, at 81,696 to 82,259
_RS02230
Position (kb) 78
79
80
81 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 77.505 kb on - strand at 77.523 kb on + strand at 77.624 kb on + strand, within SGL_RS02205 at 77.657 kb on + strand, within SGL_RS02205 at 77.708 kb on - strand, within SGL_RS02205 at 77.905 kb on - strand at 78.242 kb on - strand at 78.580 kb on - strand at 78.585 kb on - strand at 78.609 kb on + strand at 78.806 kb on + strand, within SGL_RS02220 at 78.806 kb on + strand, within SGL_RS02220 at 78.840 kb on - strand, within SGL_RS02220 at 79.137 kb on + strand, within SGL_RS02220 at 79.147 kb on + strand, within SGL_RS02220 at 79.380 kb on + strand, within SGL_RS02220 at 79.526 kb on - strand, within SGL_RS02220 at 79.667 kb on + strand, within SGL_RS02220 at 79.958 kb on + strand, within SGL_RS02220 at 80.094 kb on + strand, within SGL_RS02220 at 80.221 kb on + strand, within SGL_RS02220 at 80.288 kb on - strand, within SGL_RS02220 at 81.516 kb on - strand, within SGL_RS02225 at 81.654 kb on + strand at 81.777 kb on - strand, within SGL_RS02230
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 77,505 - -0.0 77,523 + -0.1 77,624 + SGL_RS02205 0.39 -0.0 77,657 + SGL_RS02205 0.49 -0.0 77,708 - SGL_RS02205 0.64 +0.7 77,905 - -0.0 78,242 - -0.1 78,580 - -2.6 78,585 - -2.5 78,609 + -0.3 78,806 + SGL_RS02220 0.15 -2.1 78,806 + SGL_RS02220 0.15 -3.6 78,840 - SGL_RS02220 0.16 -3.2 79,137 + SGL_RS02220 0.29 -3.2 79,147 + SGL_RS02220 0.29 -1.3 79,380 + SGL_RS02220 0.39 -2.7 79,526 - SGL_RS02220 0.45 -2.2 79,667 + SGL_RS02220 0.51 +0.1 79,958 + SGL_RS02220 0.64 -0.5 80,094 + SGL_RS02220 0.70 -3.8 80,221 + SGL_RS02220 0.75 -3.5 80,288 - SGL_RS02220 0.78 -3.7 81,516 - SGL_RS02225 0.77 -2.7 81,654 + +0.3 81,777 - SGL_RS02230 0.14 +0.0
Or see this region's nucleotide sequence