Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00315 and SGL_RS00320 are separated by 663 nucleotides SGL_RS00320 and SGL_RS19365 are separated by 153 nucleotides SGL_RS19365 and SGL_RS00330 are separated by 186 nucleotides
SGL_RS00315: SGL_RS00315 - hypothetical protein, at 69,335 to 70,810
_RS00315
SGL_RS00320: SGL_RS00320 - helix-turn-helix transcriptional regulator, at 71,474 to 71,818
_RS00320
SGL_RS19365: SGL_RS19365 - hypothetical protein, at 71,972 to 72,238
_RS19365
SGL_RS00330: SGL_RS00330 - hypothetical protein, at 72,425 to 73,159
_RS00330
Position (kb) 71
72 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 70.882 kb on + strand at 71.536 kb on - strand, within SGL_RS00320 at 71.571 kb on - strand, within SGL_RS00320 at 71.651 kb on - strand, within SGL_RS00320 at 71.766 kb on + strand, within SGL_RS00320 at 71.774 kb on - strand, within SGL_RS00320 at 71.855 kb on - strand at 71.855 kb on - strand at 71.939 kb on + strand at 72.181 kb on - strand, within SGL_RS19365 at 72.215 kb on + strand at 72.229 kb on - strand at 72.284 kb on + strand at 72.333 kb on + strand at 72.341 kb on - strand at 72.452 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 70,882 + +0.3 71,536 - SGL_RS00320 0.18 -4.1 71,571 - SGL_RS00320 0.28 -2.4 71,651 - SGL_RS00320 0.51 -2.8 71,766 + SGL_RS00320 0.85 -5.3 71,774 - SGL_RS00320 0.87 -4.4 71,855 - -0.3 71,855 - -0.0 71,939 + +0.1 72,181 - SGL_RS19365 0.78 -0.1 72,215 + -0.1 72,229 - +0.1 72,284 + +0.1 72,333 + +0.0 72,341 - +0.1 72,452 - +0.1
Or see this region's nucleotide sequence