Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14090 and SGL_RS19040 are separated by 126 nucleotides SGL_RS19040 and SGL_RS14100 overlap by 14 nucleotides SGL_RS14100 and SGL_RS14105 are separated by 38 nucleotides
SGL_RS14090: SGL_RS14090 - murein biosynthesis integral membrane protein MurJ, at 2,620,798 to 2,622,399
_RS14090
SGL_RS19040: SGL_RS19040 - hypothetical protein, at 2,622,526 to 2,622,981
_RS19040
SGL_RS14100: SGL_RS14100 - gamma-glutamylcyclotransferase, at 2,622,968 to 2,623,402
_RS14100
SGL_RS14105: SGL_RS14105 - 2-succinylbenzoate--CoA ligase, at 2,623,441 to 2,624,907
_RS14105
Position (kb)
2622
2623 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 2622.392 kb on + strand at 2622.544 kb on + strand at 2622.628 kb on - strand, within SGL_RS19040 at 2622.631 kb on - strand, within SGL_RS19040 at 2622.748 kb on + strand, within SGL_RS19040 at 2622.840 kb on - strand, within SGL_RS19040 at 2622.966 kb on - strand at 2623.080 kb on + strand, within SGL_RS14100 at 2623.085 kb on + strand, within SGL_RS14100 at 2623.236 kb on - strand, within SGL_RS14100 at 2623.247 kb on + strand, within SGL_RS14100 at 2623.370 kb on - strand at 2623.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 2,622,392 + -1.1 2,622,544 + +0.3 2,622,628 - SGL_RS19040 0.22 -4.8 2,622,631 - SGL_RS19040 0.23 -6.7 2,622,748 + SGL_RS19040 0.49 -3.9 2,622,840 - SGL_RS19040 0.69 -7.2 2,622,966 - -5.1 2,623,080 + SGL_RS14100 0.26 -6.8 2,623,085 + SGL_RS14100 0.27 -7.1 2,623,236 - SGL_RS14100 0.62 -5.2 2,623,247 + SGL_RS14100 0.64 -5.2 2,623,370 - -5.7 2,623,404 - +0.1
Or see this region's nucleotide sequence