Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18345 and SGL_RS20050 are separated by 5 nucleotides SGL_RS20050 and SGL_RS18350 are separated by 25 nucleotides SGL_RS18350 and SGL_RS18355 are separated by 111 nucleotides
SGL_RS18345: SGL_RS18345 - trans-splicing intein-formed DNA polymerase III subunit alpha N-terminal partner DnaE-N, at 3,561,946 to 3,564,639
_RS18345
SGL_RS20050: SGL_RS20050 - hypothetical protein, at 3,564,645 to 3,564,812
_RS20050
SGL_RS18350: SGL_RS18350 - translation elongation factor 4, at 3,564,838 to 3,566,649
_RS18350
SGL_RS18355: SGL_RS18355 - glutathione S-transferase family protein, at 3,566,761 to 3,567,720
_RS18355
Position (kb)
3564
3565
3566
3567 Strain fitness (log2 ratio)
-1
0
1 at 3564.591 kb on - strand at 3564.693 kb on - strand, within SGL_RS20050 at 3565.025 kb on - strand, within SGL_RS18350 at 3565.052 kb on - strand, within SGL_RS18350 at 3565.053 kb on - strand, within SGL_RS18350 at 3565.132 kb on - strand, within SGL_RS18350 at 3565.191 kb on + strand, within SGL_RS18350 at 3565.197 kb on + strand, within SGL_RS18350 at 3565.688 kb on + strand, within SGL_RS18350 at 3565.688 kb on + strand, within SGL_RS18350 at 3565.718 kb on - strand, within SGL_RS18350 at 3565.807 kb on - strand, within SGL_RS18350 at 3565.824 kb on + strand, within SGL_RS18350 at 3565.832 kb on - strand, within SGL_RS18350 at 3566.005 kb on + strand, within SGL_RS18350 at 3566.115 kb on + strand, within SGL_RS18350 at 3566.115 kb on + strand, within SGL_RS18350 at 3566.115 kb on + strand, within SGL_RS18350 at 3566.134 kb on - strand, within SGL_RS18350 at 3566.303 kb on - strand, within SGL_RS18350 at 3566.336 kb on - strand, within SGL_RS18350 at 3566.591 kb on + strand at 3566.599 kb on - strand at 3566.758 kb on + strand at 3566.813 kb on + strand at 3566.938 kb on + strand, within SGL_RS18355 at 3566.938 kb on + strand, within SGL_RS18355 at 3566.951 kb on - strand, within SGL_RS18355 at 3567.062 kb on + strand, within SGL_RS18355 at 3567.062 kb on + strand, within SGL_RS18355 at 3567.062 kb on + strand, within SGL_RS18355 at 3567.070 kb on - strand, within SGL_RS18355 at 3567.112 kb on + strand, within SGL_RS18355 at 3567.173 kb on + strand, within SGL_RS18355 at 3567.381 kb on + strand, within SGL_RS18355 at 3567.510 kb on + strand, within SGL_RS18355 at 3567.597 kb on + strand, within SGL_RS18355 at 3567.628 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 3,564,591 - -0.5 3,564,693 - SGL_RS20050 0.29 +0.2 3,565,025 - SGL_RS18350 0.10 +1.2 3,565,052 - SGL_RS18350 0.12 +0.6 3,565,053 - SGL_RS18350 0.12 +0.6 3,565,132 - SGL_RS18350 0.16 +0.7 3,565,191 + SGL_RS18350 0.19 +0.7 3,565,197 + SGL_RS18350 0.20 +1.6 3,565,688 + SGL_RS18350 0.47 +0.5 3,565,688 + SGL_RS18350 0.47 +0.4 3,565,718 - SGL_RS18350 0.49 +0.5 3,565,807 - SGL_RS18350 0.53 +0.4 3,565,824 + SGL_RS18350 0.54 -0.4 3,565,832 - SGL_RS18350 0.55 +0.6 3,566,005 + SGL_RS18350 0.64 +0.4 3,566,115 + SGL_RS18350 0.70 +1.2 3,566,115 + SGL_RS18350 0.70 +0.5 3,566,115 + SGL_RS18350 0.70 +0.7 3,566,134 - SGL_RS18350 0.72 +0.8 3,566,303 - SGL_RS18350 0.81 +0.5 3,566,336 - SGL_RS18350 0.83 -0.9 3,566,591 + +0.2 3,566,599 - +1.1 3,566,758 + +0.2 3,566,813 + -0.1 3,566,938 + SGL_RS18355 0.18 -0.1 3,566,938 + SGL_RS18355 0.18 +0.3 3,566,951 - SGL_RS18355 0.20 -0.1 3,567,062 + SGL_RS18355 0.31 +0.1 3,567,062 + SGL_RS18355 0.31 +0.2 3,567,062 + SGL_RS18355 0.31 +0.1 3,567,070 - SGL_RS18355 0.32 +0.3 3,567,112 + SGL_RS18355 0.37 -0.3 3,567,173 + SGL_RS18355 0.43 +0.2 3,567,381 + SGL_RS18355 0.65 +0.2 3,567,510 + SGL_RS18355 0.78 -0.1 3,567,597 + SGL_RS18355 0.87 -0.4 3,567,628 - -0.5
Or see this region's nucleotide sequence