Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16640 and SGL_RS16645 are separated by 48 nucleotides SGL_RS16645 and SGL_RS16650 overlap by 1 nucleotides SGL_RS16650 and SGL_RS16655 overlap by 4 nucleotides
SGL_RS16640: SGL_RS16640 - ABC transporter substrate-binding protein, at 3,188,483 to 3,189,748
_RS16640
SGL_RS16645: SGL_RS16645 - sugar ABC transporter permease, at 3,189,797 to 3,190,768
_RS16645
SGL_RS16650: SGL_RS16650 - carbohydrate ABC transporter permease, at 3,190,768 to 3,191,664
_RS16650
SGL_RS16655: SGL_RS16655 - phytoene/squalene synthase family protein, at 3,191,661 to 3,192,476
_RS16655
Position (kb)
3189
3190
3191 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3188.836 kb on + strand, within SGL_RS16640 at 3188.836 kb on + strand, within SGL_RS16640 at 3188.836 kb on + strand, within SGL_RS16640 at 3188.889 kb on + strand, within SGL_RS16640 at 3189.005 kb on - strand, within SGL_RS16640 at 3189.075 kb on - strand, within SGL_RS16640 at 3189.120 kb on + strand, within SGL_RS16640 at 3189.128 kb on - strand, within SGL_RS16640 at 3189.240 kb on - strand, within SGL_RS16640 at 3189.297 kb on + strand, within SGL_RS16640 at 3189.344 kb on + strand, within SGL_RS16640 at 3189.401 kb on + strand, within SGL_RS16640 at 3189.403 kb on - strand, within SGL_RS16640 at 3189.413 kb on - strand, within SGL_RS16640 at 3189.584 kb on - strand, within SGL_RS16640 at 3189.691 kb on - strand at 3189.930 kb on - strand, within SGL_RS16645 at 3190.122 kb on - strand, within SGL_RS16645 at 3190.136 kb on - strand, within SGL_RS16645 at 3190.222 kb on - strand, within SGL_RS16645 at 3190.313 kb on + strand, within SGL_RS16645 at 3190.430 kb on + strand, within SGL_RS16645 at 3190.453 kb on + strand, within SGL_RS16645 at 3190.532 kb on - strand, within SGL_RS16645 at 3190.855 kb on + strand at 3190.889 kb on + strand, within SGL_RS16650 at 3190.945 kb on - strand, within SGL_RS16650 at 3190.992 kb on + strand, within SGL_RS16650 at 3191.221 kb on + strand, within SGL_RS16650 at 3191.250 kb on + strand, within SGL_RS16650 at 3191.555 kb on - strand, within SGL_RS16650 at 3191.590 kb on - strand at 3191.689 kb on + strand at 3191.716 kb on + strand at 3191.724 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 3,188,836 + SGL_RS16640 0.28 -1.6 3,188,836 + SGL_RS16640 0.28 -1.9 3,188,836 + SGL_RS16640 0.28 -1.5 3,188,889 + SGL_RS16640 0.32 -0.9 3,189,005 - SGL_RS16640 0.41 -1.2 3,189,075 - SGL_RS16640 0.47 -1.3 3,189,120 + SGL_RS16640 0.50 -1.5 3,189,128 - SGL_RS16640 0.51 -1.5 3,189,240 - SGL_RS16640 0.60 -1.0 3,189,297 + SGL_RS16640 0.64 -1.6 3,189,344 + SGL_RS16640 0.68 -1.2 3,189,401 + SGL_RS16640 0.73 -1.4 3,189,403 - SGL_RS16640 0.73 -1.1 3,189,413 - SGL_RS16640 0.73 -1.2 3,189,584 - SGL_RS16640 0.87 -0.8 3,189,691 - -1.5 3,189,930 - SGL_RS16645 0.14 -0.8 3,190,122 - SGL_RS16645 0.33 -1.3 3,190,136 - SGL_RS16645 0.35 -1.4 3,190,222 - SGL_RS16645 0.44 -1.4 3,190,313 + SGL_RS16645 0.53 -1.6 3,190,430 + SGL_RS16645 0.65 -1.5 3,190,453 + SGL_RS16645 0.67 -1.0 3,190,532 - SGL_RS16645 0.76 -1.6 3,190,855 + -1.2 3,190,889 + SGL_RS16650 0.13 -1.2 3,190,945 - SGL_RS16650 0.20 -0.9 3,190,992 + SGL_RS16650 0.25 -1.1 3,191,221 + SGL_RS16650 0.51 -1.4 3,191,250 + SGL_RS16650 0.54 -1.6 3,191,555 - SGL_RS16650 0.88 -2.8 3,191,590 - -0.1 3,191,689 + -4.2 3,191,716 + -2.8 3,191,724 - -4.6
Or see this region's nucleotide sequence