Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16630 and SGL_RS20180 are separated by 61 nucleotides SGL_RS20180 and SGL_RS16640 are separated by 255 nucleotides SGL_RS16640 and SGL_RS16645 are separated by 48 nucleotides
SGL_RS16630: SGL_RS16630 - MBL fold metallo-hydrolase, at 3,186,379 to 3,188,043
_RS16630
SGL_RS20180: SGL_RS20180 - membrane protein, at 3,188,105 to 3,188,227
_RS20180
SGL_RS16640: SGL_RS16640 - ABC transporter substrate-binding protein, at 3,188,483 to 3,189,748
_RS16640
SGL_RS16645: SGL_RS16645 - sugar ABC transporter permease, at 3,189,797 to 3,190,768
_RS16645
Position (kb)
3188
3189
3190 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 3187.598 kb on - strand, within SGL_RS16630 at 3187.902 kb on + strand at 3187.915 kb on + strand at 3187.933 kb on - strand at 3187.947 kb on + strand at 3187.952 kb on + strand at 3187.977 kb on - strand at 3188.062 kb on + strand at 3188.202 kb on - strand, within SGL_RS20180 at 3188.279 kb on + strand at 3188.320 kb on - strand at 3188.544 kb on + strand at 3188.544 kb on + strand at 3188.552 kb on - strand at 3188.552 kb on - strand at 3188.557 kb on - strand at 3188.602 kb on - strand at 3188.633 kb on + strand, within SGL_RS16640 at 3188.638 kb on - strand, within SGL_RS16640 at 3188.658 kb on + strand, within SGL_RS16640 at 3188.721 kb on - strand, within SGL_RS16640 at 3188.836 kb on + strand, within SGL_RS16640 at 3188.836 kb on + strand, within SGL_RS16640 at 3188.836 kb on + strand, within SGL_RS16640 at 3188.889 kb on + strand, within SGL_RS16640 at 3189.005 kb on - strand, within SGL_RS16640 at 3189.075 kb on - strand, within SGL_RS16640 at 3189.120 kb on + strand, within SGL_RS16640 at 3189.128 kb on - strand, within SGL_RS16640 at 3189.240 kb on - strand, within SGL_RS16640 at 3189.297 kb on + strand, within SGL_RS16640 at 3189.344 kb on + strand, within SGL_RS16640 at 3189.401 kb on + strand, within SGL_RS16640 at 3189.403 kb on - strand, within SGL_RS16640 at 3189.413 kb on - strand, within SGL_RS16640 at 3189.584 kb on - strand, within SGL_RS16640 at 3189.691 kb on - strand at 3189.930 kb on - strand, within SGL_RS16645 at 3190.122 kb on - strand, within SGL_RS16645 at 3190.136 kb on - strand, within SGL_RS16645 at 3190.222 kb on - strand, within SGL_RS16645 at 3190.313 kb on + strand, within SGL_RS16645 at 3190.430 kb on + strand, within SGL_RS16645 at 3190.453 kb on + strand, within SGL_RS16645 at 3190.532 kb on - strand, within SGL_RS16645
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 3,187,598 - SGL_RS16630 0.73 -6.1 3,187,902 + -8.0 3,187,915 + -8.0 3,187,933 - -6.0 3,187,947 + -5.6 3,187,952 + -8.2 3,187,977 - -5.8 3,188,062 + -0.0 3,188,202 - SGL_RS20180 0.79 -3.7 3,188,279 + +0.3 3,188,320 - -0.4 3,188,544 + -0.9 3,188,544 + -0.7 3,188,552 - -0.6 3,188,552 - -1.3 3,188,557 - -1.0 3,188,602 - -0.8 3,188,633 + SGL_RS16640 0.12 -1.5 3,188,638 - SGL_RS16640 0.12 -1.1 3,188,658 + SGL_RS16640 0.14 -1.9 3,188,721 - SGL_RS16640 0.19 -1.8 3,188,836 + SGL_RS16640 0.28 -1.6 3,188,836 + SGL_RS16640 0.28 -1.9 3,188,836 + SGL_RS16640 0.28 -1.5 3,188,889 + SGL_RS16640 0.32 -0.9 3,189,005 - SGL_RS16640 0.41 -1.2 3,189,075 - SGL_RS16640 0.47 -1.3 3,189,120 + SGL_RS16640 0.50 -1.5 3,189,128 - SGL_RS16640 0.51 -1.5 3,189,240 - SGL_RS16640 0.60 -1.0 3,189,297 + SGL_RS16640 0.64 -1.6 3,189,344 + SGL_RS16640 0.68 -1.2 3,189,401 + SGL_RS16640 0.73 -1.4 3,189,403 - SGL_RS16640 0.73 -1.1 3,189,413 - SGL_RS16640 0.73 -1.2 3,189,584 - SGL_RS16640 0.87 -0.8 3,189,691 - -1.5 3,189,930 - SGL_RS16645 0.14 -0.8 3,190,122 - SGL_RS16645 0.33 -1.3 3,190,136 - SGL_RS16645 0.35 -1.4 3,190,222 - SGL_RS16645 0.44 -1.4 3,190,313 + SGL_RS16645 0.53 -1.6 3,190,430 + SGL_RS16645 0.65 -1.5 3,190,453 + SGL_RS16645 0.67 -1.0 3,190,532 - SGL_RS16645 0.76 -1.6
Or see this region's nucleotide sequence