Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16360 and SGL_RS16365 are separated by 100 nucleotides SGL_RS16365 and SGL_RS16370 are separated by 88 nucleotides SGL_RS16370 and SGL_RS16375 are separated by 92 nucleotides
SGL_RS16360: SGL_RS16360 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 3,117,042 to 3,118,091
_RS16360
SGL_RS16365: SGL_RS16365 - PetM family cytochrome b6-f complex subunit 7, at 3,118,192 to 3,118,302
_RS16365
SGL_RS16370: SGL_RS16370 - tRNA guanosine(34) transglycosylase Tgt, at 3,118,391 to 3,119,515
_RS16370
SGL_RS16375: SGL_RS16375 - hypothetical protein, at 3,119,608 to 3,121,176
_RS16375
Position (kb)
3118
3119
3120 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3117.460 kb on + strand, within SGL_RS16360 at 3117.500 kb on - strand, within SGL_RS16360 at 3117.547 kb on + strand, within SGL_RS16360 at 3117.555 kb on - strand, within SGL_RS16360 at 3117.555 kb on - strand, within SGL_RS16360 at 3117.583 kb on - strand, within SGL_RS16360 at 3117.886 kb on - strand, within SGL_RS16360 at 3117.944 kb on + strand, within SGL_RS16360 at 3117.953 kb on - strand, within SGL_RS16360 at 3118.076 kb on + strand at 3118.101 kb on + strand at 3118.131 kb on - strand at 3118.215 kb on + strand, within SGL_RS16365 at 3118.252 kb on + strand, within SGL_RS16365 at 3118.258 kb on + strand, within SGL_RS16365 at 3118.263 kb on + strand, within SGL_RS16365 at 3118.330 kb on - strand at 3118.330 kb on - strand at 3118.330 kb on - strand at 3118.453 kb on - strand at 3118.537 kb on - strand, within SGL_RS16370 at 3118.568 kb on + strand, within SGL_RS16370 at 3118.582 kb on + strand, within SGL_RS16370 at 3118.582 kb on + strand, within SGL_RS16370 at 3118.707 kb on - strand, within SGL_RS16370 at 3118.769 kb on - strand, within SGL_RS16370 at 3118.829 kb on - strand, within SGL_RS16370 at 3118.931 kb on - strand, within SGL_RS16370 at 3118.950 kb on + strand, within SGL_RS16370 at 3118.968 kb on - strand, within SGL_RS16370 at 3119.024 kb on + strand, within SGL_RS16370 at 3119.024 kb on + strand, within SGL_RS16370 at 3119.025 kb on + strand, within SGL_RS16370 at 3119.025 kb on + strand, within SGL_RS16370 at 3119.032 kb on - strand, within SGL_RS16370 at 3119.032 kb on - strand, within SGL_RS16370 at 3119.032 kb on - strand, within SGL_RS16370 at 3119.032 kb on - strand, within SGL_RS16370 at 3119.068 kb on + strand, within SGL_RS16370 at 3119.101 kb on + strand, within SGL_RS16370 at 3119.165 kb on + strand, within SGL_RS16370 at 3119.218 kb on + strand, within SGL_RS16370 at 3119.218 kb on - strand, within SGL_RS16370 at 3119.252 kb on - strand, within SGL_RS16370 at 3119.252 kb on - strand, within SGL_RS16370 at 3119.323 kb on + strand, within SGL_RS16370 at 3119.423 kb on + strand at 3119.423 kb on - strand at 3119.438 kb on + strand at 3119.502 kb on - strand at 3119.517 kb on - strand at 3119.585 kb on - strand at 3119.679 kb on - strand at 3119.821 kb on + strand, within SGL_RS16375 at 3119.847 kb on - strand, within SGL_RS16375 at 3119.882 kb on - strand, within SGL_RS16375 at 3119.996 kb on + strand, within SGL_RS16375 at 3120.055 kb on - strand, within SGL_RS16375 at 3120.361 kb on + strand, within SGL_RS16375
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 3,117,460 + SGL_RS16360 0.40 -2.2 3,117,500 - SGL_RS16360 0.44 -4.7 3,117,547 + SGL_RS16360 0.48 -3.5 3,117,555 - SGL_RS16360 0.49 -2.0 3,117,555 - SGL_RS16360 0.49 -3.4 3,117,583 - SGL_RS16360 0.52 -3.2 3,117,886 - SGL_RS16360 0.80 -4.5 3,117,944 + SGL_RS16360 0.86 -3.5 3,117,953 - SGL_RS16360 0.87 -3.0 3,118,076 + -0.4 3,118,101 + +0.4 3,118,131 - -0.1 3,118,215 + SGL_RS16365 0.21 -0.3 3,118,252 + SGL_RS16365 0.54 +0.4 3,118,258 + SGL_RS16365 0.59 +0.5 3,118,263 + SGL_RS16365 0.64 +0.3 3,118,330 - -1.0 3,118,330 - -1.3 3,118,330 - -0.8 3,118,453 - -2.5 3,118,537 - SGL_RS16370 0.13 -3.2 3,118,568 + SGL_RS16370 0.16 -3.5 3,118,582 + SGL_RS16370 0.17 -2.9 3,118,582 + SGL_RS16370 0.17 -3.2 3,118,707 - SGL_RS16370 0.28 -3.3 3,118,769 - SGL_RS16370 0.34 -3.7 3,118,829 - SGL_RS16370 0.39 -3.2 3,118,931 - SGL_RS16370 0.48 -2.0 3,118,950 + SGL_RS16370 0.50 -2.9 3,118,968 - SGL_RS16370 0.51 -2.3 3,119,024 + SGL_RS16370 0.56 -3.5 3,119,024 + SGL_RS16370 0.56 -3.5 3,119,025 + SGL_RS16370 0.56 -3.4 3,119,025 + SGL_RS16370 0.56 -4.6 3,119,032 - SGL_RS16370 0.57 -2.4 3,119,032 - SGL_RS16370 0.57 -3.0 3,119,032 - SGL_RS16370 0.57 -3.2 3,119,032 - SGL_RS16370 0.57 -1.4 3,119,068 + SGL_RS16370 0.60 -3.0 3,119,101 + SGL_RS16370 0.63 -2.8 3,119,165 + SGL_RS16370 0.69 -3.8 3,119,218 + SGL_RS16370 0.74 -3.3 3,119,218 - SGL_RS16370 0.74 -2.9 3,119,252 - SGL_RS16370 0.77 -2.2 3,119,252 - SGL_RS16370 0.77 -3.4 3,119,323 + SGL_RS16370 0.83 -2.3 3,119,423 + -2.6 3,119,423 - -3.0 3,119,438 + -3.4 3,119,502 - -1.7 3,119,517 - -0.3 3,119,585 - -0.3 3,119,679 - +0.0 3,119,821 + SGL_RS16375 0.14 -0.2 3,119,847 - SGL_RS16375 0.15 -0.0 3,119,882 - SGL_RS16375 0.17 +0.0 3,119,996 + SGL_RS16375 0.25 +0.0 3,120,055 - SGL_RS16375 0.28 -0.2 3,120,361 + SGL_RS16375 0.48 +0.3
Or see this region's nucleotide sequence