Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14920 and SGL_RS14925 are separated by 225 nucleotides SGL_RS14925 and SGL_RS14930 are separated by 34 nucleotides SGL_RS14930 and SGL_RS14935 are separated by 19 nucleotides SGL_RS14935 and SGL_RS14940 are separated by 70 nucleotides
SGL_RS14920: SGL_RS14920 - cation:proton antiporter, at 2,801,140 to 2,803,035
_RS14920
SGL_RS14925: SGL_RS14925 - tRNA-Cys, at 2,803,261 to 2,803,331
_RS14925
SGL_RS14930: SGL_RS14930 - type I methionyl aminopeptidase, at 2,803,366 to 2,804,208
_RS14930
SGL_RS14935: SGL_RS14935 - ferredoxin:thioredoxin reductase, at 2,804,228 to 2,804,584
_RS14935
SGL_RS14940: SGL_RS14940 - alpha/beta hydrolase, at 2,804,655 to 2,805,692
_RS14940
Position (kb)
2803
2804
2805 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 2802.527 kb on + strand, within SGL_RS14920 at 2802.582 kb on - strand, within SGL_RS14920 at 2802.780 kb on + strand, within SGL_RS14920 at 2802.904 kb on + strand at 2803.071 kb on + strand at 2803.141 kb on - strand at 2803.563 kb on + strand, within SGL_RS14930 at 2803.566 kb on - strand, within SGL_RS14930 at 2803.625 kb on - strand, within SGL_RS14930 at 2803.646 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2804.037 kb on - strand, within SGL_RS14930 at 2804.164 kb on + strand at 2804.171 kb on + strand at 2804.172 kb on - strand at 2804.641 kb on - strand at 2804.664 kb on - strand at 2804.811 kb on - strand, within SGL_RS14940 at 2804.906 kb on + strand, within SGL_RS14940 at 2804.950 kb on - strand, within SGL_RS14940 at 2805.171 kb on + strand, within SGL_RS14940 at 2805.179 kb on - strand, within SGL_RS14940 at 2805.179 kb on - strand, within SGL_RS14940
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 2,802,527 + SGL_RS14920 0.73 -4.5 2,802,582 - SGL_RS14920 0.76 -3.3 2,802,780 + SGL_RS14920 0.86 +0.3 2,802,904 + -3.4 2,803,071 + +0.2 2,803,141 - +0.1 2,803,563 + SGL_RS14930 0.23 -6.4 2,803,566 - SGL_RS14930 0.24 -5.6 2,803,625 - SGL_RS14930 0.31 -7.3 2,803,646 + SGL_RS14930 0.33 -5.3 2,803,781 + SGL_RS14930 0.49 -5.5 2,803,781 + SGL_RS14930 0.49 -3.9 2,803,781 + SGL_RS14930 0.49 -3.1 2,803,781 + SGL_RS14930 0.49 -5.1 2,804,037 - SGL_RS14930 0.80 -5.9 2,804,164 + -6.2 2,804,171 + -7.8 2,804,172 - -6.5 2,804,641 - -4.0 2,804,664 - +0.5 2,804,811 - SGL_RS14940 0.15 +0.3 2,804,906 + SGL_RS14940 0.24 +0.5 2,804,950 - SGL_RS14940 0.28 -0.1 2,805,171 + SGL_RS14940 0.50 +0.4 2,805,179 - SGL_RS14940 0.50 +0.2 2,805,179 - SGL_RS14940 0.50 +0.2
Or see this region's nucleotide sequence