Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13170 and SGL_RS13175 are separated by 145 nucleotides SGL_RS13175 and SGL_RS13180 are separated by 200 nucleotides SGL_RS13180 and SGL_RS13185 are separated by 21 nucleotides
SGL_RS13170: SGL_RS13170 - histidine kinase, at 2,421,536 to 2,422,687
_RS13170
SGL_RS13175: SGL_RS13175 - GerMN domain-containing protein, at 2,422,833 to 2,423,420
_RS13175
SGL_RS13180: SGL_RS13180 - triose-phosphate isomerase, at 2,423,621 to 2,424,349
_RS13180
SGL_RS13185: SGL_RS13185 - AI-2E family transporter, at 2,424,371 to 2,425,390
_RS13185
Position (kb)
2422
2423
2424 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 2422.181 kb on - strand, within SGL_RS13170 at 2422.183 kb on - strand, within SGL_RS13170 at 2422.207 kb on - strand, within SGL_RS13170 at 2422.231 kb on + strand, within SGL_RS13170 at 2422.609 kb on - strand at 2422.727 kb on + strand at 2422.762 kb on + strand at 2422.804 kb on - strand at 2422.917 kb on + strand, within SGL_RS13175 at 2423.019 kb on + strand, within SGL_RS13175 at 2423.048 kb on + strand, within SGL_RS13175 at 2423.090 kb on - strand, within SGL_RS13175 at 2423.326 kb on - strand, within SGL_RS13175 at 2423.381 kb on - strand at 2423.562 kb on - strand at 2424.098 kb on - strand, within SGL_RS13180 at 2424.375 kb on + strand at 2424.392 kb on + strand at 2424.392 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 2,422,181 - SGL_RS13170 0.56 +0.2 2,422,183 - SGL_RS13170 0.56 -0.3 2,422,207 - SGL_RS13170 0.58 -0.2 2,422,231 + SGL_RS13170 0.60 +0.5 2,422,609 - +0.4 2,422,727 + +0.3 2,422,762 + -0.1 2,422,804 - -0.3 2,422,917 + SGL_RS13175 0.14 -4.5 2,423,019 + SGL_RS13175 0.32 -5.6 2,423,048 + SGL_RS13175 0.37 -8.0 2,423,090 - SGL_RS13175 0.44 -5.7 2,423,326 - SGL_RS13175 0.84 -7.1 2,423,381 - -6.3 2,423,562 - +0.0 2,424,098 - SGL_RS13180 0.65 -1.3 2,424,375 + -1.9 2,424,392 + -2.9 2,424,392 + -3.3
Or see this region's nucleotide sequence