Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12540 and SGL_RS12545 are separated by 460 nucleotides SGL_RS12545 and SGL_RS12550 are separated by 9 nucleotides
SGL_RS12540: SGL_RS12540 - RNA polymerase sigma factor, RpoD/SigA family, at 2,269,126 to 2,270,163
_RS12540
SGL_RS12545: SGL_RS12545 - GTPase Era, at 2,270,624 to 2,271,571
_RS12545
SGL_RS12550: SGL_RS12550 - hybrid sensor histidine kinase/response regulator, at 2,271,581 to 2,274,868
_RS12550
Position (kb)
2270
2271
2272 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 2269.750 kb on - strand, within SGL_RS12540 at 2269.859 kb on - strand, within SGL_RS12540 at 2270.051 kb on - strand, within SGL_RS12540 at 2270.087 kb on + strand at 2270.197 kb on - strand at 2270.204 kb on + strand at 2270.204 kb on + strand at 2270.214 kb on + strand at 2270.317 kb on + strand at 2270.319 kb on - strand at 2270.463 kb on + strand at 2270.554 kb on - strand at 2270.710 kb on - strand at 2271.062 kb on - strand, within SGL_RS12545 at 2271.118 kb on - strand, within SGL_RS12545 at 2271.157 kb on - strand, within SGL_RS12545 at 2271.232 kb on + strand, within SGL_RS12545 at 2271.684 kb on - strand at 2271.751 kb on - strand at 2271.813 kb on - strand at 2272.087 kb on - strand, within SGL_RS12550 at 2272.331 kb on - strand, within SGL_RS12550 at 2272.353 kb on + strand, within SGL_RS12550 at 2272.389 kb on - strand, within SGL_RS12550
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 2,269,750 - SGL_RS12540 0.60 -6.2 2,269,859 - SGL_RS12540 0.71 -8.0 2,270,051 - SGL_RS12540 0.89 -6.4 2,270,087 + -4.3 2,270,197 - -5.4 2,270,204 + -7.3 2,270,204 + -8.2 2,270,214 + -5.2 2,270,317 + -3.3 2,270,319 - -4.8 2,270,463 + -0.7 2,270,554 - +0.1 2,270,710 - -2.2 2,271,062 - SGL_RS12545 0.46 -3.6 2,271,118 - SGL_RS12545 0.52 -3.0 2,271,157 - SGL_RS12545 0.56 -2.0 2,271,232 + SGL_RS12545 0.64 -4.0 2,271,684 - -1.8 2,271,751 - -3.5 2,271,813 - -3.0 2,272,087 - SGL_RS12550 0.15 -2.5 2,272,331 - SGL_RS12550 0.23 -2.8 2,272,353 + SGL_RS12550 0.23 -2.0 2,272,389 - SGL_RS12550 0.25 -1.1
Or see this region's nucleotide sequence