Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12030 and SGL_RS12035 are separated by 35 nucleotides SGL_RS12035 and SGL_RS12040 are separated by 97 nucleotides SGL_RS12040 and SGL_RS12045 are separated by 100 nucleotides SGL_RS12045 and SGL_RS12050 are separated by 302 nucleotides
SGL_RS12030: SGL_RS12030 - extracellular solute-binding protein, at 2,150,247 to 2,151,401
_RS12030
SGL_RS12035: SGL_RS12035 - tRNA-Thr, at 2,151,437 to 2,151,508
_RS12035
SGL_RS12040: SGL_RS12040 - RdgB/HAM1 family non-canonical purine NTP pyrophosphatase, at 2,151,606 to 2,152,190
_RS12040
SGL_RS12045: SGL_RS12045 - P-II family nitrogen regulator, at 2,152,291 to 2,152,629
_RS12045
SGL_RS12050: SGL_RS12050 - DUF1517 domain-containing protein, at 2,152,932 to 2,153,933
_RS12050
Position (kb)
2151
2152
2153 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2150.658 kb on - strand, within SGL_RS12030 at 2150.694 kb on + strand, within SGL_RS12030 at 2150.749 kb on - strand, within SGL_RS12030 at 2150.863 kb on + strand, within SGL_RS12030 at 2150.953 kb on - strand, within SGL_RS12030 at 2151.119 kb on + strand, within SGL_RS12030 at 2151.139 kb on - strand, within SGL_RS12030 at 2151.216 kb on - strand, within SGL_RS12030 at 2151.339 kb on + strand at 2151.396 kb on + strand at 2151.425 kb on - strand at 2151.532 kb on + strand at 2151.700 kb on - strand, within SGL_RS12040 at 2151.881 kb on - strand, within SGL_RS12040 at 2151.906 kb on + strand, within SGL_RS12040 at 2151.921 kb on - strand, within SGL_RS12040 at 2151.950 kb on + strand, within SGL_RS12040 at 2152.008 kb on - strand, within SGL_RS12040 at 2152.029 kb on - strand, within SGL_RS12040 at 2152.207 kb on - strand at 2152.332 kb on - strand, within SGL_RS12045 at 2152.420 kb on + strand, within SGL_RS12045 at 2152.502 kb on + strand, within SGL_RS12045 at 2152.829 kb on - strand at 2152.843 kb on + strand at 2152.851 kb on - strand at 2152.951 kb on + strand at 2153.077 kb on + strand, within SGL_RS12050
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 2,150,658 - SGL_RS12030 0.36 -3.0 2,150,694 + SGL_RS12030 0.39 -2.2 2,150,749 - SGL_RS12030 0.43 -1.6 2,150,863 + SGL_RS12030 0.53 -5.3 2,150,953 - SGL_RS12030 0.61 -3.2 2,151,119 + SGL_RS12030 0.75 -3.5 2,151,139 - SGL_RS12030 0.77 -2.3 2,151,216 - SGL_RS12030 0.84 -4.4 2,151,339 + -3.6 2,151,396 + +0.1 2,151,425 - +0.0 2,151,532 + +0.1 2,151,700 - SGL_RS12040 0.16 +0.2 2,151,881 - SGL_RS12040 0.47 +0.0 2,151,906 + SGL_RS12040 0.51 +0.2 2,151,921 - SGL_RS12040 0.54 +0.1 2,151,950 + SGL_RS12040 0.59 -0.3 2,152,008 - SGL_RS12040 0.69 +0.1 2,152,029 - SGL_RS12040 0.72 -0.3 2,152,207 - -1.2 2,152,332 - SGL_RS12045 0.12 -3.2 2,152,420 + SGL_RS12045 0.38 -3.2 2,152,502 + SGL_RS12045 0.62 -3.5 2,152,829 - -1.3 2,152,843 + -0.1 2,152,851 - -0.4 2,152,951 + -0.9 2,153,077 + SGL_RS12050 0.14 -0.7
Or see this region's nucleotide sequence