Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11945 and SGL_RS11950 are separated by 3 nucleotides SGL_RS11950 and SGL_RS11955 are separated by 179 nucleotides
SGL_RS11945: SGL_RS11945 - sirohydrochlorin chelatase, at 2,132,715 to 2,133,545
_RS11945
SGL_RS11950: SGL_RS11950 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 2,133,549 to 2,134,022
_RS11950
SGL_RS11955: SGL_RS11955 - aminomethyl-transferring glycine dehydrogenase, at 2,134,202 to 2,137,153
_RS11955
Position (kb)
2133
2134
2135 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2132.608 kb on + strand at 2132.655 kb on + strand at 2132.663 kb on - strand at 2132.786 kb on + strand at 2132.799 kb on + strand, within SGL_RS11945 at 2132.957 kb on + strand, within SGL_RS11945 at 2132.963 kb on + strand, within SGL_RS11945 at 2132.963 kb on + strand, within SGL_RS11945 at 2132.971 kb on - strand, within SGL_RS11945 at 2132.984 kb on + strand, within SGL_RS11945 at 2133.053 kb on + strand, within SGL_RS11945 at 2133.061 kb on - strand, within SGL_RS11945 at 2133.086 kb on - strand, within SGL_RS11945 at 2133.096 kb on + strand, within SGL_RS11945 at 2133.101 kb on + strand, within SGL_RS11945 at 2133.109 kb on - strand, within SGL_RS11945 at 2133.109 kb on - strand, within SGL_RS11945 at 2133.168 kb on + strand, within SGL_RS11945 at 2133.464 kb on - strand at 2133.490 kb on + strand at 2133.546 kb on + strand at 2133.554 kb on - strand at 2133.588 kb on + strand at 2133.596 kb on - strand at 2133.596 kb on - strand at 2133.692 kb on + strand, within SGL_RS11950 at 2133.786 kb on + strand, within SGL_RS11950 at 2133.864 kb on - strand, within SGL_RS11950 at 2133.872 kb on - strand, within SGL_RS11950 at 2133.884 kb on - strand, within SGL_RS11950 at 2133.886 kb on + strand, within SGL_RS11950 at 2133.931 kb on + strand, within SGL_RS11950 at 2134.094 kb on + strand at 2134.094 kb on + strand at 2134.094 kb on + strand at 2134.094 kb on + strand at 2134.094 kb on + strand at 2134.094 kb on + strand at 2134.094 kb on + strand at 2134.102 kb on - strand at 2134.102 kb on - strand at 2134.102 kb on - strand at 2134.244 kb on - strand at 2134.251 kb on + strand at 2134.781 kb on + strand, within SGL_RS11955 at 2134.839 kb on + strand, within SGL_RS11955 at 2135.015 kb on - strand, within SGL_RS11955
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 2,132,608 + +0.9 2,132,655 + +0.5 2,132,663 - +0.6 2,132,786 + -5.1 2,132,799 + SGL_RS11945 0.10 -5.9 2,132,957 + SGL_RS11945 0.29 -5.8 2,132,963 + SGL_RS11945 0.30 -4.8 2,132,963 + SGL_RS11945 0.30 -4.4 2,132,971 - SGL_RS11945 0.31 -6.5 2,132,984 + SGL_RS11945 0.32 -4.4 2,133,053 + SGL_RS11945 0.41 -5.1 2,133,061 - SGL_RS11945 0.42 -5.9 2,133,086 - SGL_RS11945 0.45 -6.2 2,133,096 + SGL_RS11945 0.46 -6.7 2,133,101 + SGL_RS11945 0.46 -3.3 2,133,109 - SGL_RS11945 0.47 -5.8 2,133,109 - SGL_RS11945 0.47 -5.2 2,133,168 + SGL_RS11945 0.55 -6.7 2,133,464 - -4.3 2,133,490 + -6.6 2,133,546 + +0.2 2,133,554 - +0.5 2,133,588 + -5.4 2,133,596 - -5.3 2,133,596 - -5.6 2,133,692 + SGL_RS11950 0.30 -4.6 2,133,786 + SGL_RS11950 0.50 -5.3 2,133,864 - SGL_RS11950 0.66 -5.6 2,133,872 - SGL_RS11950 0.68 -3.6 2,133,884 - SGL_RS11950 0.71 -5.5 2,133,886 + SGL_RS11950 0.71 -5.6 2,133,931 + SGL_RS11950 0.81 -4.1 2,134,094 + +0.2 2,134,094 + +0.2 2,134,094 + -0.2 2,134,094 + -0.4 2,134,094 + -0.2 2,134,094 + -0.3 2,134,094 + +0.1 2,134,102 - +0.0 2,134,102 - +0.0 2,134,102 - +0.6 2,134,244 - -2.3 2,134,251 + -4.4 2,134,781 + SGL_RS11955 0.20 -3.8 2,134,839 + SGL_RS11955 0.22 -2.0 2,135,015 - SGL_RS11955 0.28 -3.2
Or see this region's nucleotide sequence