Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11450 and SGL_RS11455 are separated by 30 nucleotides SGL_RS11455 and SGL_RS11460 are separated by 153 nucleotides SGL_RS11460 and SGL_RS11465 are separated by 112 nucleotides SGL_RS11465 and SGL_RS11470 are separated by 26 nucleotides
SGL_RS11450: SGL_RS11450 - cysteine--tRNA ligase, at 2,026,675 to 2,028,126
_RS11450
SGL_RS11455: SGL_RS11455 - Maf family nucleotide pyrophosphatase, at 2,028,157 to 2,028,744
_RS11455
SGL_RS11460: SGL_RS11460 - type II CAAX endopeptidase family protein, at 2,028,898 to 2,030,421
_RS11460
SGL_RS11465: SGL_RS11465 - hypothetical protein, at 2,030,534 to 2,030,974
_RS11465
SGL_RS11470: SGL_RS11470 - carotenoid oxygenase family protein, at 2,031,001 to 2,032,473
_RS11470
Position (kb)
2028
2029
2030
2031 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 2028.301 kb on + strand, within SGL_RS11455 at 2028.302 kb on + strand, within SGL_RS11455 at 2028.302 kb on + strand, within SGL_RS11455 at 2028.328 kb on - strand, within SGL_RS11455 at 2028.339 kb on - strand, within SGL_RS11455 at 2028.407 kb on - strand, within SGL_RS11455 at 2028.501 kb on - strand, within SGL_RS11455 at 2028.503 kb on + strand, within SGL_RS11455 at 2028.503 kb on + strand, within SGL_RS11455 at 2028.511 kb on - strand, within SGL_RS11455 at 2028.511 kb on - strand, within SGL_RS11455 at 2028.592 kb on - strand, within SGL_RS11455 at 2028.609 kb on - strand, within SGL_RS11455 at 2028.625 kb on - strand, within SGL_RS11455 at 2028.714 kb on - strand at 2028.868 kb on + strand at 2028.968 kb on + strand at 2028.968 kb on + strand at 2028.976 kb on - strand at 2029.126 kb on - strand, within SGL_RS11460 at 2029.168 kb on - strand, within SGL_RS11460 at 2029.199 kb on - strand, within SGL_RS11460 at 2029.287 kb on + strand, within SGL_RS11460 at 2029.352 kb on - strand, within SGL_RS11460 at 2029.410 kb on - strand, within SGL_RS11460 at 2029.548 kb on + strand, within SGL_RS11460 at 2029.819 kb on + strand, within SGL_RS11460 at 2029.819 kb on + strand, within SGL_RS11460 at 2029.863 kb on - strand, within SGL_RS11460 at 2029.952 kb on + strand, within SGL_RS11460 at 2030.000 kb on - strand, within SGL_RS11460 at 2030.026 kb on + strand, within SGL_RS11460 at 2030.198 kb on - strand, within SGL_RS11460 at 2030.208 kb on - strand, within SGL_RS11460 at 2030.243 kb on + strand, within SGL_RS11460 at 2030.254 kb on - strand, within SGL_RS11460 at 2030.500 kb on - strand at 2030.505 kb on - strand at 2030.599 kb on + strand, within SGL_RS11465 at 2031.022 kb on + strand at 2031.085 kb on - strand at 2031.297 kb on + strand, within SGL_RS11470 at 2031.300 kb on - strand, within SGL_RS11470
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 2,028,301 + SGL_RS11455 0.24 +0.4 2,028,302 + SGL_RS11455 0.25 +1.9 2,028,302 + SGL_RS11455 0.25 +0.6 2,028,328 - SGL_RS11455 0.29 +0.2 2,028,339 - SGL_RS11455 0.31 +0.5 2,028,407 - SGL_RS11455 0.43 +0.5 2,028,501 - SGL_RS11455 0.59 -0.2 2,028,503 + SGL_RS11455 0.59 -1.0 2,028,503 + SGL_RS11455 0.59 +0.2 2,028,511 - SGL_RS11455 0.60 -0.1 2,028,511 - SGL_RS11455 0.60 +0.8 2,028,592 - SGL_RS11455 0.74 +0.4 2,028,609 - SGL_RS11455 0.77 +0.4 2,028,625 - SGL_RS11455 0.80 +0.3 2,028,714 - -0.2 2,028,868 + -2.1 2,028,968 + -7.7 2,028,968 + -6.7 2,028,976 - -4.3 2,029,126 - SGL_RS11460 0.15 -5.5 2,029,168 - SGL_RS11460 0.18 -4.2 2,029,199 - SGL_RS11460 0.20 -7.1 2,029,287 + SGL_RS11460 0.26 -5.9 2,029,352 - SGL_RS11460 0.30 -5.0 2,029,410 - SGL_RS11460 0.34 -6.5 2,029,548 + SGL_RS11460 0.43 -6.9 2,029,819 + SGL_RS11460 0.60 -3.5 2,029,819 + SGL_RS11460 0.60 -8.1 2,029,863 - SGL_RS11460 0.63 -3.8 2,029,952 + SGL_RS11460 0.69 -7.6 2,030,000 - SGL_RS11460 0.72 -4.6 2,030,026 + SGL_RS11460 0.74 -7.4 2,030,198 - SGL_RS11460 0.85 -6.3 2,030,208 - SGL_RS11460 0.86 -5.2 2,030,243 + SGL_RS11460 0.88 -6.8 2,030,254 - SGL_RS11460 0.89 -7.3 2,030,500 - +0.2 2,030,505 - +0.3 2,030,599 + SGL_RS11465 0.15 -0.2 2,031,022 + +0.5 2,031,085 - -0.1 2,031,297 + SGL_RS11470 0.20 +0.6 2,031,300 - SGL_RS11470 0.20 -0.9
Or see this region's nucleotide sequence