Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11160 and SGL_RS11165 are separated by 17 nucleotides SGL_RS11165 and SGL_RS11170 are separated by 196 nucleotides SGL_RS11170 and SGL_RS11175 are separated by 349 nucleotides
SGL_RS11160: SGL_RS11160 - ParA family protein, at 1,962,705 to 1,965,467
_RS11160
SGL_RS11165: SGL_RS11165 - hypothetical protein, at 1,965,485 to 1,965,949
_RS11165
SGL_RS11170: SGL_RS11170 - L-glutamate gamma-semialdehyde dehydrogenase, at 1,966,146 to 1,969,118
_RS11170
SGL_RS11175: SGL_RS11175 - hypothetical protein, at 1,969,468 to 1,970,430
_RS11175
Position (kb)
1966
1967
1968
1969
1970 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1965.598 kb on - strand, within SGL_RS11165 at 1965.720 kb on - strand, within SGL_RS11165 at 1966.101 kb on + strand at 1966.196 kb on + strand at 1966.204 kb on - strand at 1966.430 kb on - strand at 1966.504 kb on + strand, within SGL_RS11170 at 1966.529 kb on + strand, within SGL_RS11170 at 1966.591 kb on + strand, within SGL_RS11170 at 1966.591 kb on + strand, within SGL_RS11170 at 1966.599 kb on - strand, within SGL_RS11170 at 1966.599 kb on - strand, within SGL_RS11170 at 1966.616 kb on - strand, within SGL_RS11170 at 1966.623 kb on + strand, within SGL_RS11170 at 1966.624 kb on + strand, within SGL_RS11170 at 1966.660 kb on + strand, within SGL_RS11170 at 1966.668 kb on - strand, within SGL_RS11170 at 1966.797 kb on + strand, within SGL_RS11170 at 1966.797 kb on + strand, within SGL_RS11170 at 1966.808 kb on - strand, within SGL_RS11170 at 1967.011 kb on + strand, within SGL_RS11170 at 1967.050 kb on - strand, within SGL_RS11170 at 1967.050 kb on - strand, within SGL_RS11170 at 1967.050 kb on - strand, within SGL_RS11170 at 1967.050 kb on - strand, within SGL_RS11170 at 1967.051 kb on - strand, within SGL_RS11170 at 1967.113 kb on + strand, within SGL_RS11170 at 1967.131 kb on - strand, within SGL_RS11170 at 1967.159 kb on + strand, within SGL_RS11170 at 1967.278 kb on - strand, within SGL_RS11170 at 1967.368 kb on - strand, within SGL_RS11170 at 1967.371 kb on + strand, within SGL_RS11170 at 1967.436 kb on - strand, within SGL_RS11170 at 1967.497 kb on + strand, within SGL_RS11170 at 1967.507 kb on + strand, within SGL_RS11170 at 1967.507 kb on + strand, within SGL_RS11170 at 1967.520 kb on - strand, within SGL_RS11170 at 1967.575 kb on - strand, within SGL_RS11170 at 1967.605 kb on + strand, within SGL_RS11170 at 1967.636 kb on + strand, within SGL_RS11170 at 1967.660 kb on - strand, within SGL_RS11170 at 1967.670 kb on + strand, within SGL_RS11170 at 1967.724 kb on - strand, within SGL_RS11170 at 1967.878 kb on + strand, within SGL_RS11170 at 1967.891 kb on - strand, within SGL_RS11170 at 1967.919 kb on + strand, within SGL_RS11170 at 1967.919 kb on + strand, within SGL_RS11170 at 1967.927 kb on - strand, within SGL_RS11170 at 1967.927 kb on - strand, within SGL_RS11170 at 1967.927 kb on - strand, within SGL_RS11170 at 1967.933 kb on - strand, within SGL_RS11170 at 1968.000 kb on + strand, within SGL_RS11170 at 1968.005 kb on + strand, within SGL_RS11170 at 1968.007 kb on + strand, within SGL_RS11170 at 1968.008 kb on - strand, within SGL_RS11170 at 1968.082 kb on + strand, within SGL_RS11170 at 1968.215 kb on + strand, within SGL_RS11170 at 1968.227 kb on + strand, within SGL_RS11170 at 1968.282 kb on + strand, within SGL_RS11170 at 1968.360 kb on + strand, within SGL_RS11170 at 1968.368 kb on - strand, within SGL_RS11170 at 1968.443 kb on - strand, within SGL_RS11170 at 1968.706 kb on - strand, within SGL_RS11170 at 1968.961 kb on - strand at 1969.047 kb on + strand at 1969.237 kb on - strand at 1969.553 kb on + strand at 1969.576 kb on + strand, within SGL_RS11175 at 1969.584 kb on - strand, within SGL_RS11175 at 1969.604 kb on + strand, within SGL_RS11175 at 1969.659 kb on + strand, within SGL_RS11175 at 1969.660 kb on + strand, within SGL_RS11175 at 1969.803 kb on + strand, within SGL_RS11175
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 1,965,598 - SGL_RS11165 0.24 -0.2 1,965,720 - SGL_RS11165 0.51 -1.2 1,966,101 + -0.5 1,966,196 + -4.6 1,966,204 - -6.3 1,966,430 - -1.2 1,966,504 + SGL_RS11170 0.12 -5.7 1,966,529 + SGL_RS11170 0.13 -3.3 1,966,591 + SGL_RS11170 0.15 -4.3 1,966,591 + SGL_RS11170 0.15 -3.2 1,966,599 - SGL_RS11170 0.15 -3.7 1,966,599 - SGL_RS11170 0.15 -3.7 1,966,616 - SGL_RS11170 0.16 -2.7 1,966,623 + SGL_RS11170 0.16 -5.4 1,966,624 + SGL_RS11170 0.16 -3.5 1,966,660 + SGL_RS11170 0.17 -3.6 1,966,668 - SGL_RS11170 0.18 -3.9 1,966,797 + SGL_RS11170 0.22 -4.8 1,966,797 + SGL_RS11170 0.22 -3.5 1,966,808 - SGL_RS11170 0.22 -4.2 1,967,011 + SGL_RS11170 0.29 -3.5 1,967,050 - SGL_RS11170 0.30 -3.0 1,967,050 - SGL_RS11170 0.30 -3.0 1,967,050 - SGL_RS11170 0.30 -3.6 1,967,050 - SGL_RS11170 0.30 -3.5 1,967,051 - SGL_RS11170 0.30 -5.6 1,967,113 + SGL_RS11170 0.33 -2.7 1,967,131 - SGL_RS11170 0.33 -5.7 1,967,159 + SGL_RS11170 0.34 -3.7 1,967,278 - SGL_RS11170 0.38 -3.6 1,967,368 - SGL_RS11170 0.41 -4.8 1,967,371 + SGL_RS11170 0.41 -5.3 1,967,436 - SGL_RS11170 0.43 -3.2 1,967,497 + SGL_RS11170 0.45 -3.3 1,967,507 + SGL_RS11170 0.46 -4.1 1,967,507 + SGL_RS11170 0.46 -3.2 1,967,520 - SGL_RS11170 0.46 -2.7 1,967,575 - SGL_RS11170 0.48 -3.8 1,967,605 + SGL_RS11170 0.49 -3.5 1,967,636 + SGL_RS11170 0.50 -3.3 1,967,660 - SGL_RS11170 0.51 -3.1 1,967,670 + SGL_RS11170 0.51 -3.5 1,967,724 - SGL_RS11170 0.53 -4.0 1,967,878 + SGL_RS11170 0.58 -3.2 1,967,891 - SGL_RS11170 0.59 -4.9 1,967,919 + SGL_RS11170 0.60 -3.3 1,967,919 + SGL_RS11170 0.60 -3.8 1,967,927 - SGL_RS11170 0.60 -3.2 1,967,927 - SGL_RS11170 0.60 -3.9 1,967,927 - SGL_RS11170 0.60 -3.8 1,967,933 - SGL_RS11170 0.60 -3.9 1,968,000 + SGL_RS11170 0.62 -4.6 1,968,005 + SGL_RS11170 0.63 -3.2 1,968,007 + SGL_RS11170 0.63 -2.6 1,968,008 - SGL_RS11170 0.63 -3.9 1,968,082 + SGL_RS11170 0.65 -3.6 1,968,215 + SGL_RS11170 0.70 -4.2 1,968,227 + SGL_RS11170 0.70 -3.7 1,968,282 + SGL_RS11170 0.72 -7.0 1,968,360 + SGL_RS11170 0.74 -3.3 1,968,368 - SGL_RS11170 0.75 -4.4 1,968,443 - SGL_RS11170 0.77 -4.2 1,968,706 - SGL_RS11170 0.86 -3.5 1,968,961 - -2.4 1,969,047 + -3.8 1,969,237 - -0.5 1,969,553 + +0.1 1,969,576 + SGL_RS11175 0.11 -0.3 1,969,584 - SGL_RS11175 0.12 -0.2 1,969,604 + SGL_RS11175 0.14 -0.5 1,969,659 + SGL_RS11175 0.20 +0.1 1,969,660 + SGL_RS11175 0.20 -0.0 1,969,803 + SGL_RS11175 0.35 +0.1
Or see this region's nucleotide sequence