Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS19610 and SGL_RS11075 are separated by 64 nucleotides SGL_RS11075 and SGL_RS11080 are separated by 143 nucleotides SGL_RS11080 and SGL_RS11085 are separated by 145 nucleotides
SGL_RS19610: SGL_RS19610 - hypothetical protein, at 1,944,345 to 1,944,521
_RS19610
SGL_RS11075: SGL_RS11075 - ABC-2 family transporter protein, at 1,944,586 to 1,945,374
_RS11075
SGL_RS11080: SGL_RS11080 - glycerol-3-phosphate dehydrogenase, at 1,945,518 to 1,947,179
_RS11080
SGL_RS11085: SGL_RS11085 - glucosylglycerol-phosphate synthase, at 1,947,325 to 1,948,824
_RS11085
Position (kb) 1945
1946
1947
1948 Strain fitness (log2 ratio) -9
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 1944.542 kb on - strand at 1944.572 kb on - strand at 1944.933 kb on + strand, within SGL_RS11075 at 1944.991 kb on - strand, within SGL_RS11075 at 1945.109 kb on - strand, within SGL_RS11075 at 1945.109 kb on - strand, within SGL_RS11075 at 1945.162 kb on + strand, within SGL_RS11075 at 1945.170 kb on - strand, within SGL_RS11075 at 1945.175 kb on + strand, within SGL_RS11075 at 1945.325 kb on - strand at 1945.801 kb on + strand, within SGL_RS11080 at 1945.960 kb on - strand, within SGL_RS11080 at 1945.972 kb on - strand, within SGL_RS11080 at 1945.995 kb on - strand, within SGL_RS11080 at 1945.995 kb on - strand, within SGL_RS11080 at 1945.995 kb on - strand, within SGL_RS11080 at 1946.113 kb on - strand, within SGL_RS11080 at 1946.134 kb on + strand, within SGL_RS11080 at 1946.134 kb on + strand, within SGL_RS11080 at 1946.134 kb on + strand, within SGL_RS11080 at 1946.142 kb on - strand, within SGL_RS11080 at 1946.258 kb on - strand, within SGL_RS11080 at 1946.260 kb on - strand, within SGL_RS11080 at 1946.369 kb on - strand, within SGL_RS11080 at 1946.394 kb on + strand, within SGL_RS11080 at 1946.484 kb on + strand, within SGL_RS11080 at 1946.634 kb on - strand, within SGL_RS11080 at 1946.634 kb on - strand, within SGL_RS11080 at 1946.658 kb on - strand, within SGL_RS11080 at 1946.697 kb on + strand, within SGL_RS11080 at 1946.877 kb on + strand, within SGL_RS11080 at 1947.068 kb on + strand at 1947.093 kb on + strand at 1947.101 kb on - strand at 1947.209 kb on - strand at 1947.358 kb on + strand at 1947.514 kb on + strand, within SGL_RS11085 at 1947.565 kb on + strand, within SGL_RS11085 at 1947.573 kb on - strand, within SGL_RS11085 at 1947.573 kb on - strand, within SGL_RS11085 at 1947.619 kb on + strand, within SGL_RS11085 at 1947.646 kb on - strand, within SGL_RS11085 at 1947.689 kb on - strand, within SGL_RS11085 at 1947.691 kb on + strand, within SGL_RS11085 at 1947.691 kb on + strand, within SGL_RS11085 at 1947.691 kb on + strand, within SGL_RS11085 at 1947.699 kb on - strand, within SGL_RS11085 at 1947.701 kb on + strand, within SGL_RS11085 at 1947.711 kb on - strand, within SGL_RS11085 at 1947.837 kb on + strand, within SGL_RS11085 at 1947.850 kb on + strand, within SGL_RS11085 at 1948.083 kb on + strand, within SGL_RS11085
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 1,944,542 - +0.2 1,944,572 - +0.1 1,944,933 + SGL_RS11075 0.44 -0.3 1,944,991 - SGL_RS11075 0.51 +0.1 1,945,109 - SGL_RS11075 0.66 -0.1 1,945,109 - SGL_RS11075 0.66 -0.2 1,945,162 + SGL_RS11075 0.73 -0.1 1,945,170 - SGL_RS11075 0.74 -0.9 1,945,175 + SGL_RS11075 0.75 -0.1 1,945,325 - +0.3 1,945,801 + SGL_RS11080 0.17 -3.5 1,945,960 - SGL_RS11080 0.27 -8.1 1,945,972 - SGL_RS11080 0.27 -7.4 1,945,995 - SGL_RS11080 0.29 -9.2 1,945,995 - SGL_RS11080 0.29 -5.4 1,945,995 - SGL_RS11080 0.29 -8.3 1,946,113 - SGL_RS11080 0.36 -6.4 1,946,134 + SGL_RS11080 0.37 -6.9 1,946,134 + SGL_RS11080 0.37 -6.7 1,946,134 + SGL_RS11080 0.37 -6.6 1,946,142 - SGL_RS11080 0.38 -4.6 1,946,258 - SGL_RS11080 0.45 -6.3 1,946,260 - SGL_RS11080 0.45 -6.0 1,946,369 - SGL_RS11080 0.51 -6.9 1,946,394 + SGL_RS11080 0.53 -4.1 1,946,484 + SGL_RS11080 0.58 -2.7 1,946,634 - SGL_RS11080 0.67 -5.2 1,946,634 - SGL_RS11080 0.67 -6.2 1,946,658 - SGL_RS11080 0.69 -4.9 1,946,697 + SGL_RS11080 0.71 -4.6 1,946,877 + SGL_RS11080 0.82 -7.5 1,947,068 + -5.2 1,947,093 + -5.3 1,947,101 - -7.5 1,947,209 - -4.2 1,947,358 + -6.7 1,947,514 + SGL_RS11085 0.13 -5.7 1,947,565 + SGL_RS11085 0.16 -3.2 1,947,573 - SGL_RS11085 0.17 -4.6 1,947,573 - SGL_RS11085 0.17 -3.8 1,947,619 + SGL_RS11085 0.20 -6.7 1,947,646 - SGL_RS11085 0.21 -3.9 1,947,689 - SGL_RS11085 0.24 -6.1 1,947,691 + SGL_RS11085 0.24 -5.3 1,947,691 + SGL_RS11085 0.24 -7.5 1,947,691 + SGL_RS11085 0.24 -7.9 1,947,699 - SGL_RS11085 0.25 -4.5 1,947,701 + SGL_RS11085 0.25 -8.1 1,947,711 - SGL_RS11085 0.26 -6.1 1,947,837 + SGL_RS11085 0.34 -4.4 1,947,850 + SGL_RS11085 0.35 -5.9 1,948,083 + SGL_RS11085 0.51 -7.1
Or see this region's nucleotide sequence