Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09705 and SGL_RS09710 are separated by 147 nucleotides SGL_RS09710 and SGL_RS09715 are separated by 183 nucleotides SGL_RS09715 and SGL_RS09720 are separated by 7 nucleotides
SGL_RS09705: SGL_RS09705 - F0F1 ATP synthase subunit beta, at 1,666,932 to 1,668,383
_RS09705
SGL_RS09710: SGL_RS09710 - ATP synthase F1 subunit epsilon, at 1,668,531 to 1,668,941
_RS09710
SGL_RS09715: SGL_RS09715 - pitrilysin family protein, at 1,669,125 to 1,670,642
_RS09715
SGL_RS09720: SGL_RS09720 - beta-ketoacyl-ACP synthase, at 1,670,650 to 1,671,864
_RS09720
Position (kb)
1669
1670
1671 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1668.936 kb on + strand at 1668.956 kb on - strand at 1668.967 kb on - strand at 1669.300 kb on + strand, within SGL_RS09715 at 1669.624 kb on + strand, within SGL_RS09715 at 1669.647 kb on + strand, within SGL_RS09715 at 1669.783 kb on + strand, within SGL_RS09715 at 1669.808 kb on - strand, within SGL_RS09715 at 1669.875 kb on - strand, within SGL_RS09715 at 1669.889 kb on - strand, within SGL_RS09715 at 1669.956 kb on + strand, within SGL_RS09715 at 1670.079 kb on + strand, within SGL_RS09715 at 1670.097 kb on - strand, within SGL_RS09715 at 1670.098 kb on + strand, within SGL_RS09715 at 1670.189 kb on - strand, within SGL_RS09715 at 1670.211 kb on + strand, within SGL_RS09715 at 1670.211 kb on + strand, within SGL_RS09715 at 1670.211 kb on + strand, within SGL_RS09715 at 1670.211 kb on + strand, within SGL_RS09715 at 1670.219 kb on - strand, within SGL_RS09715 at 1670.219 kb on - strand, within SGL_RS09715 at 1670.305 kb on + strand, within SGL_RS09715 at 1670.342 kb on - strand, within SGL_RS09715 at 1670.586 kb on + strand at 1670.587 kb on + strand at 1670.688 kb on + strand at 1670.722 kb on + strand at 1670.725 kb on - strand at 1670.730 kb on - strand at 1670.884 kb on + strand, within SGL_RS09720 at 1670.956 kb on - strand, within SGL_RS09720 at 1671.004 kb on - strand, within SGL_RS09720 at 1671.084 kb on - strand, within SGL_RS09720 at 1671.134 kb on + strand, within SGL_RS09720 at 1671.142 kb on - strand, within SGL_RS09720 at 1671.170 kb on + strand, within SGL_RS09720 at 1671.310 kb on - strand, within SGL_RS09720 at 1671.530 kb on + strand, within SGL_RS09720 at 1671.626 kb on + strand, within SGL_RS09720 at 1671.626 kb on + strand, within SGL_RS09720 at 1671.634 kb on - strand, within SGL_RS09720 at 1671.634 kb on - strand, within SGL_RS09720
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 1,668,936 + -0.6 1,668,956 - -0.4 1,668,967 - -0.1 1,669,300 + SGL_RS09715 0.12 -4.1 1,669,624 + SGL_RS09715 0.33 -4.0 1,669,647 + SGL_RS09715 0.34 -5.3 1,669,783 + SGL_RS09715 0.43 -3.1 1,669,808 - SGL_RS09715 0.45 -2.8 1,669,875 - SGL_RS09715 0.49 -6.5 1,669,889 - SGL_RS09715 0.50 -4.2 1,669,956 + SGL_RS09715 0.55 -4.4 1,670,079 + SGL_RS09715 0.63 -2.4 1,670,097 - SGL_RS09715 0.64 -5.1 1,670,098 + SGL_RS09715 0.64 -3.4 1,670,189 - SGL_RS09715 0.70 -4.6 1,670,211 + SGL_RS09715 0.72 -5.6 1,670,211 + SGL_RS09715 0.72 -4.9 1,670,211 + SGL_RS09715 0.72 -6.5 1,670,211 + SGL_RS09715 0.72 -6.4 1,670,219 - SGL_RS09715 0.72 -4.5 1,670,219 - SGL_RS09715 0.72 -4.9 1,670,305 + SGL_RS09715 0.78 -5.4 1,670,342 - SGL_RS09715 0.80 -4.2 1,670,586 + -3.9 1,670,587 + -1.3 1,670,688 + +0.2 1,670,722 + -3.1 1,670,725 - -3.2 1,670,730 - -3.5 1,670,884 + SGL_RS09720 0.19 -3.1 1,670,956 - SGL_RS09720 0.25 -3.5 1,671,004 - SGL_RS09720 0.29 -1.6 1,671,084 - SGL_RS09720 0.36 -2.1 1,671,134 + SGL_RS09720 0.40 -1.9 1,671,142 - SGL_RS09720 0.40 -3.2 1,671,170 + SGL_RS09720 0.43 -1.6 1,671,310 - SGL_RS09720 0.54 -3.8 1,671,530 + SGL_RS09720 0.72 +0.5 1,671,626 + SGL_RS09720 0.80 -2.4 1,671,626 + SGL_RS09720 0.80 -2.6 1,671,634 - SGL_RS09720 0.81 -3.5 1,671,634 - SGL_RS09720 0.81 -2.9
Or see this region's nucleotide sequence