Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08800 and SGL_RS08805 are separated by 167 nucleotides SGL_RS08805 and SGL_RS08810 are separated by 285 nucleotides SGL_RS08810 and SGL_RS08815 overlap by 4 nucleotides SGL_RS08815 and SGL_RS08820 are separated by 98 nucleotides
SGL_RS08800: SGL_RS08800 - DUF3153 domain-containing protein, at 1,478,988 to 1,479,782
_RS08800
SGL_RS08805: SGL_RS08805 - hypothetical protein, at 1,479,950 to 1,480,177
_RS08805
SGL_RS08810: SGL_RS08810 - metal ABC transporter permease, at 1,480,463 to 1,481,383
_RS08810
SGL_RS08815: SGL_RS08815 - metal ABC transporter ATP-binding protein, at 1,481,380 to 1,482,162
_RS08815
SGL_RS08820: SGL_RS08820 - metal ABC transporter substrate-binding protein, at 1,482,261 to 1,483,253
_RS08820
Position (kb)
1480
1481
1482 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1479.777 kb on + strand at 1479.777 kb on + strand at 1479.882 kb on - strand at 1480.474 kb on - strand at 1480.573 kb on - strand, within SGL_RS08810 at 1480.632 kb on - strand, within SGL_RS08810 at 1480.661 kb on + strand, within SGL_RS08810 at 1480.663 kb on + strand, within SGL_RS08810 at 1480.789 kb on + strand, within SGL_RS08810 at 1480.810 kb on - strand, within SGL_RS08810 at 1481.041 kb on - strand, within SGL_RS08810 at 1481.183 kb on + strand, within SGL_RS08810 at 1481.188 kb on + strand, within SGL_RS08810 at 1481.188 kb on + strand, within SGL_RS08810 at 1481.191 kb on - strand, within SGL_RS08810 at 1481.196 kb on - strand, within SGL_RS08810 at 1481.279 kb on - strand, within SGL_RS08810 at 1481.338 kb on + strand at 1481.358 kb on + strand at 1481.488 kb on - strand, within SGL_RS08815 at 1481.688 kb on - strand, within SGL_RS08815 at 1482.068 kb on + strand, within SGL_RS08815 at 1482.153 kb on - strand at 1482.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 1,479,777 + -0.5 1,479,777 + -0.3 1,479,882 - -0.3 1,480,474 - -2.2 1,480,573 - SGL_RS08810 0.12 -5.4 1,480,632 - SGL_RS08810 0.18 -5.2 1,480,661 + SGL_RS08810 0.21 -3.3 1,480,663 + SGL_RS08810 0.22 -6.0 1,480,789 + SGL_RS08810 0.35 -4.7 1,480,810 - SGL_RS08810 0.38 -5.5 1,481,041 - SGL_RS08810 0.63 -2.9 1,481,183 + SGL_RS08810 0.78 -5.0 1,481,188 + SGL_RS08810 0.79 -5.8 1,481,188 + SGL_RS08810 0.79 -3.4 1,481,191 - SGL_RS08810 0.79 -3.1 1,481,196 - SGL_RS08810 0.80 -5.8 1,481,279 - SGL_RS08810 0.89 -2.6 1,481,338 + -5.8 1,481,358 + -3.1 1,481,488 - SGL_RS08815 0.14 -3.9 1,481,688 - SGL_RS08815 0.39 -7.2 1,482,068 + SGL_RS08815 0.88 -6.1 1,482,153 - -3.3 1,482,206 + -2.1
Or see this region's nucleotide sequence