Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08130 and SGL_RS08135 are separated by 318 nucleotides SGL_RS08135 and SGL_RS08140 are separated by 90 nucleotides SGL_RS08140 and SGL_RS08145 overlap by 1 nucleotides
SGL_RS08130: SGL_RS08130 - Rieske 2Fe-2S domain-containing protein, at 1,335,571 to 1,336,980
_RS08130
SGL_RS08135: SGL_RS08135 - NAD(P)/FAD-dependent oxidoreductase, at 1,337,299 to 1,338,636
_RS08135
SGL_RS08140: SGL_RS08140 - hypothetical protein, at 1,338,727 to 1,339,155
_RS08140
SGL_RS08145: SGL_RS08145 - ribosomal protein S18-alanine N-acetyltransferase, at 1,339,155 to 1,339,631
_RS08145
Position (kb)
1337
1338
1339 Strain fitness (log2 ratio)
-1
0
1 at 1336.375 kb on + strand, within SGL_RS08130 at 1336.385 kb on + strand, within SGL_RS08130 at 1336.901 kb on + strand at 1337.315 kb on - strand at 1337.360 kb on + strand at 1337.361 kb on + strand at 1337.458 kb on + strand, within SGL_RS08135 at 1337.506 kb on + strand, within SGL_RS08135 at 1337.599 kb on + strand, within SGL_RS08135 at 1337.613 kb on - strand, within SGL_RS08135 at 1338.034 kb on - strand, within SGL_RS08135 at 1338.114 kb on - strand, within SGL_RS08135 at 1338.124 kb on - strand, within SGL_RS08135 at 1338.134 kb on - strand, within SGL_RS08135 at 1338.161 kb on - strand, within SGL_RS08135 at 1338.165 kb on - strand, within SGL_RS08135 at 1338.165 kb on - strand, within SGL_RS08135 at 1338.173 kb on + strand, within SGL_RS08135 at 1338.267 kb on + strand, within SGL_RS08135 at 1338.389 kb on + strand, within SGL_RS08135 at 1338.416 kb on - strand, within SGL_RS08135 at 1338.619 kb on + strand at 1338.762 kb on + strand at 1338.767 kb on - strand at 1338.835 kb on - strand, within SGL_RS08140 at 1338.856 kb on - strand, within SGL_RS08140 at 1338.863 kb on - strand, within SGL_RS08140 at 1338.863 kb on - strand, within SGL_RS08140 at 1338.870 kb on + strand, within SGL_RS08140 at 1338.923 kb on - strand, within SGL_RS08140 at 1338.933 kb on - strand, within SGL_RS08140 at 1339.033 kb on - strand, within SGL_RS08140 at 1339.201 kb on + strand at 1339.406 kb on - strand, within SGL_RS08145 at 1339.453 kb on - strand, within SGL_RS08145 at 1339.513 kb on - strand, within SGL_RS08145 at 1339.591 kb on + strand at 1339.599 kb on - strand at 1339.621 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 1,336,375 + SGL_RS08130 0.57 -0.0 1,336,385 + SGL_RS08130 0.58 +0.1 1,336,901 + -0.3 1,337,315 - +0.2 1,337,360 + -0.4 1,337,361 + +0.7 1,337,458 + SGL_RS08135 0.12 +0.1 1,337,506 + SGL_RS08135 0.15 +0.3 1,337,599 + SGL_RS08135 0.22 +0.1 1,337,613 - SGL_RS08135 0.23 -0.0 1,338,034 - SGL_RS08135 0.55 +0.3 1,338,114 - SGL_RS08135 0.61 -0.8 1,338,124 - SGL_RS08135 0.62 -0.2 1,338,134 - SGL_RS08135 0.62 +0.5 1,338,161 - SGL_RS08135 0.64 +0.6 1,338,165 - SGL_RS08135 0.65 -0.1 1,338,165 - SGL_RS08135 0.65 +0.1 1,338,173 + SGL_RS08135 0.65 +0.4 1,338,267 + SGL_RS08135 0.72 -0.2 1,338,389 + SGL_RS08135 0.81 -0.7 1,338,416 - SGL_RS08135 0.83 -0.4 1,338,619 + -0.1 1,338,762 + +1.0 1,338,767 - +0.1 1,338,835 - SGL_RS08140 0.25 +0.5 1,338,856 - SGL_RS08140 0.30 +0.3 1,338,863 - SGL_RS08140 0.32 +0.4 1,338,863 - SGL_RS08140 0.32 +0.2 1,338,870 + SGL_RS08140 0.33 -0.3 1,338,923 - SGL_RS08140 0.46 +0.1 1,338,933 - SGL_RS08140 0.48 -0.1 1,339,033 - SGL_RS08140 0.71 -0.0 1,339,201 + +0.6 1,339,406 - SGL_RS08145 0.53 +0.8 1,339,453 - SGL_RS08145 0.62 +0.4 1,339,513 - SGL_RS08145 0.75 +0.1 1,339,591 + +0.4 1,339,599 - +0.2 1,339,621 + -0.3
Or see this region's nucleotide sequence